DICP OpenIR
学科主题物理化学
Investigation of alkaloids concentration using solid phase extraction
Zhang J(张静); Liu YF(刘艳芳); Zhang FF(章飞芳); Xue XY(薛兴亚); Xu Q(徐青); Liang XM(梁鑫淼)
会议名称13th International Biotechnology Symposium & Exhibition
会议日期2008-10-12
2008-10-12
会议地点中国
其他题名生物碱的固相萃取研究
页码S104/2
部门归属十八室
主办者中国科学院、中国工程院、教育部、科技部和国家发改委
摘要Solid-phase extraction (SPE) is an effective tool for the fraction concentration and sample clean-up in the analysis of complex samples [1, 2]. In order to concentrate alkaloids from the preparation fraction of traditional Chinese medicine, many SPE columns including polymer (Oasis HLB), phenyl, CN, C18 were investigated in this work. Several important extraction parameters [3], such as sample loading flow, sample concentration, elution condition were evaluated. The recovery demonstrated that Oasis HLB and C18 were better than others. For the basic property of alkaloids, acidic elution solution was suitable for the high recovery, especially for the C18 SPE column. It can be deduced from the comparison of Oasis HLB and C18 that the adsorption capacity of C18 was more than Oasis HLB. However, the dead adsorption was occurred on C18. For Oasis HLB, the interaction between the stationary phase and alkaloids was a little weaker than C18. So the alkaloids can be easily eluted from the SPE column and had no dead adsorption. It is demonstrated that Oasis HLB was a suitable alternative for the alkaloids concentration from the preparation fraction of traditional Chinese medicine. References: [1] Jin, Y., Xue, X. Y, Zhang, F. F., Zhang, J., Shi, H., Xiao, Y. S., Ke, Y. X., Liang, X. M., 2008. Rapid prediction and optimization of concentration conditions for preparative fractions by solid-phase extraction. J. Sep. Sci. 31: 615–621 [2] Kursinszke, L., Hank, H., La''szlo'', I., Szo""ke, ''E., 2005. Simultaneous analysis of hyoscyamine, scopolamine, 6β-hydroxyhyoscyamine and apoatropine in Solanaceous hairy roots by reversed-phase high-performance liquid chromatography. J. Chromatogr. A. 1091: 32-39 [3] Tang, D. T., Liang, H. L., Zhang, L., Chen, H. L., 2007. Solid Phase Extraction of Solanesol. Chromatographis. 66: 129-131
语种中文
WOS记录号WOS:000208979400243
引用统计
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/113156
专题中国科学院大连化学物理研究所
通讯作者Liang XM(梁鑫淼)
推荐引用方式
GB/T 7714
Zhang J,Liu YF,Zhang FF,et al. Investigation of alkaloids concentration using solid phase extraction[C],2008:S104/2.
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