Subject Area物理化学
Tandem immunoaffinity columns coupled with LC-MS/MS for the determination of isoproturon and its metabolite in river water
Zhang XL(张秀莉); DieterMartens; PetraM.Krä; mer; AntoniusA.Ketrrup; Liang XM(梁鑫淼)
Conference Name13th International Biotechnology Symposium & Exhibition
Conference Date2008-10-12
Conference Place中国
Alternative Title串联免疫亲和柱与LC-MS/MS联用测定河水中异丙隆及其降解产物
Funding Organization中国科学院、中国工程院、教育部、科技部和国家发改委
AbstractIsoproturon is a widely used herbicide[1]. Its first metabolite is mono-demethyl-isoproturon (D1M-Ipo) in environmental condition. Both of them may accumulate and achieve intolerable concentrations in drinking water [2]. The maximum admissible concentration in drinking water set by the European Union is 0.1 μg/L. Thus, a sensitive and selective method could be preferred for trace analysis of isoproturon and D1M-Ipo in water. Two kind of monoclonal anti-isoproturon antibodies were entrapped in a sol-gel matrix, respectively. Depending on their affinity to isoproturon, the two immunoaffinity columns were named LIAC and HIAC. The LIAC can concentrate the isoproturon in 25mL water with 90% recovery using 40% (v/v) acetonitrile (ACN) as our previous described [3], but only with 9% recovery for D1M-Ipo. The HIAC can concentrate D1M-Ipo in 25mL water with 95.6% recovery using 40% ACN and isoproturon with 70% ACN. However, the capacity of HIAC after one time elution of 70% ACN decreased from 120ng to 50ng, that mean the HIAC can’t be reused for the concentration of isoproturon. After ensuring that isoproturon in 40% ACN will not bind on the HIAC, the LIAC was connected with HIAC by a SPE column adaptor to establish the tandem immunoaffinity extraction method. The LIAC-HIAC was combined with liquid chromatography–tandem mass spectrometry to determine isoproturon and D1M-Ipo simultaneously in surface water, and the linear range was up to 2.2 μg/l with correlation coefficient higher than 0.99 and more than 88% recoveries (n = 8). All of these data proved that tandem immunoaffinity columns were effective and reusable tool for extraction of isoproturon and D1M-Ipo from aqueous matrix with good reliability and high selectivity. [1] Tomlin, C.; (Editor) 1994. The Pesticide Manual, 10th ed. ed.; Royal Society of Chemistry and the British Crop Protection Council: Farnham, Surrey, UK, [2] Krämer, P. M.; Kremmer, E.; Forster, S.; Goodrow, M. H. 2004, Extending the Working Range of Immunoanalysis by Exploitation of Two Monoclonal Antibodies J. Agric. Food Chem. 52, 6394-6401. [3] Zhang, X. L.; Martens, D.; Krämer, P. M.; Kettrup, A. A.; Liang, X. M. 2006, Development and application of a sol–gel immunosorbent-based method for the determination of isoproturon in surface water J. Chromatogr. A 1102, 84-90.
WOS IDWOS:000208979401517
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Document Type会议论文
Corresponding AuthorLiang XM(梁鑫淼)
Recommended Citation
GB/T 7714
Zhang XL,DieterMartens,PetraM.Krä,et al. Tandem immunoaffinity columns coupled with LC-MS/MS for the determination of isoproturon and its metabolite in river water[C],2008:S436/2.
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