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学科主题: 物理化学
题名: IDENTIFICATION OF THE OROTIDINE-5’-MONOPHOSPHATE DECARBOXYLASE GENE OF THE OLEAGINOUS YEAST RHODOSPORIDIUM TORULOIDES
作者: Yang F(杨帆) ;  Zhang SF(张素芳) ;  Tang W(唐伟) ;  Zhao ZB(赵宗保)
会议名称: 13th International Biotechnology Symposium and Exhibition
会议日期: 2008-10-12
出版日期: 2008-10-12
会议地点: 中国
通讯作者: 赵宗保
ISSN: 0168-1656
部门归属: 十八室
主办者: 中国科学院,中国工程院,中国教育部,中国科技部
摘要: Rhodosporidium toruloides is an excellent microbial lipid producer of great industrial potential [1]; yet, there is no effective genetic tool for rational engineering this microorganism. Orotidine-5’-monophosphate decarboxylase (OMPdecase), usually encoded by the gene ura3, and has been widely employed as a genetic selection maker in Saccharomyces cerevisiae [2], other yeasts [3] and fungi. To develop a marker recycling system for R. toruloides, the ura3 gene of R. toruloides (Rt-ura3) was isolated using methods of degenerate PCR together with RACE. Results showed that the cDNA and genomic sequence for Rt-ura3 were 840 bp and 1017 bp, respectively, indication that the gene contained 4 extrons and 3 introns. The encoded polypeptide held an amino acid sequence of 279 residues with significant homology to those of OMPdecases from other yeasts. A shuttle vector pYES2/CT-Rt-ura3 was constructed via site-specific insertion of Rt-ura3 into the commercial vector pYES2/CT. Transformation of the vector into S. cerevisiae BY4741, a URA3-deficient yeast strain, ensured the viability of the strain on synthetic dextrose agar plate without uracil, suggesting that the isolated Rt-ura3 was functionally equivalent to the ura3 gene from S. cerevisiae. Further work is going on in this laboratory to engineer oleaginous yeasts based on this information.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113176
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Yang F,Zhang SF,Tang W,et al. IDENTIFICATION OF THE OROTIDINE-5’-MONOPHOSPHATE DECARBOXYLASE GENE OF THE OLEAGINOUS YEAST RHODOSPORIDIUM TORULOIDES[C]. 见:13th International Biotechnology Symposium and Exhibition. 中国. 2008-10-12.
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