DICP OpenIR
Subject Area物理化学
IDENTIFICATION OF THE OROTIDINE-5’-MONOPHOSPHATE DECARBOXYLASE GENE OF THE OLEAGINOUS YEAST RHODOSPORIDIUM TORULOIDES
杨帆; 张素芳; 唐伟; 赵宗保
Conference Name13th International Biotechnology Symposium and Exhibition
Conference Date2008-10-12
2008-10-12
Conference Place中国
PagesS323/2
ISSN0168-1656
Department十八室
Funding Organization中国科学院,中国工程院,中国教育部,中国科技部
Other AbstractRhodosporidium toruloides is an excellent microbial lipid producer of great industrial potential [1]; yet, there is no effective genetic tool for rational engineering this microorganism. Orotidine-5’-monophosphate decarboxylase (OMPdecase), usually encoded by the gene ura3, and has been widely employed as a genetic selection maker in Saccharomyces cerevisiae [2], other yeasts [3] and fungi. To develop a marker recycling system for R. toruloides, the ura3 gene of R. toruloides (Rt-ura3) was isolated using methods of degenerate PCR together with RACE. Results showed that the cDNA and genomic sequence for Rt-ura3 were 840 bp and 1017 bp, respectively, indication that the gene contained 4 extrons and 3 introns. The encoded polypeptide held an amino acid sequence of 279 residues with significant homology to those of OMPdecases from other yeasts. A shuttle vector pYES2/CT-Rt-ura3 was constructed via site-specific insertion of Rt-ura3 into the commercial vector pYES2/CT. Transformation of the vector into S. cerevisiae BY4741, a URA3-deficient yeast strain, ensured the viability of the strain on synthetic dextrose agar plate without uracil, suggesting that the isolated Rt-ura3 was functionally equivalent to the ura3 gene from S. cerevisiae. Further work is going on in this laboratory to engineer oleaginous yeasts based on this information.
Language中文
Document Type会议论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/113176
Collection中国科学院大连化学物理研究所
Corresponding Author赵宗保
Recommended Citation
GB/T 7714
杨帆,张素芳,唐伟,等. IDENTIFICATION OF THE OROTIDINE-5’-MONOPHOSPHATE DECARBOXYLASE GENE OF THE OLEAGINOUS YEAST RHODOSPORIDIUM TORULOIDES[C],2008:S323/2.
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