DICP OpenIR
学科主题物理化学
Cloning and characterization of the isocitrate dehydrogenase gene from oleaginous yeast Lipomyces starkeyi
Tang W(唐伟); Zhang SF(张素芳); Tan HD(谭海东); Zhao ZB(赵宗保)
会议名称13th International Biotechnology Symposium and Exhibition
会议日期2008-10-12
2008-10-12
会议地点中国
页码S359/2
ISSN0168-1656
部门归属十八室
主办者中国科学院,中国工程院,中国教育部,中国科技部
英文摘要Oleaginous yeast Lipomyces starkeyi can accumulate intracellular lipids over 65% of its cell dry weight [1]. Mitochondrial NAD+-specific isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrare to α-ketoglutarate in eukaryotic cells, an essential and rate-limiting step in citric acid cycle. Yeast S. cerevisiae IDH is composed of an octamer of four IDH1 and four IDH2 subunits [2]. Early biochemical study showed that IDH activity correlates with the lipid storage capacity [3]. In this work we cloned the full length cDNA sequence of the mitochondrial IDH1 and IDH2 gene using methods of degenerate polymerase chain reaction (PCR) and rapid amplification of cDNA ends from L. starkeyi AS 2.1560, a useful oil-producing yeast. We further determined the expression level of both lsidh1 and lsidh2 by real time PCR during the lipid storage process. Our results showed the encoded products, LsIDH1 and LsIDH2, are similar in size with a molecular weight of 40,277 and 40,708, respectively, and have high similarity with IDH sequences from other yeast species. More importantly, putative mitochondrial transit peptides have been identified at the N-terminus of each sequence, suggesting that both proteins are likely located within the mitochondria. Further gene expression analysis by real time PCR showed that both idh1 and idh2 expression decreased concurrently with the evolution of cellular lipids. Our data may be valuable for further engineering oleaginous yeasts.
语种中文
WOS记录号WOS:000208979401333
引用统计
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/113180
专题中国科学院大连化学物理研究所
通讯作者Zhao ZB(赵宗保)
推荐引用方式
GB/T 7714
Tang W,Zhang SF,Tan HD,et al. Cloning and characterization of the isocitrate dehydrogenase gene from oleaginous yeast Lipomyces starkeyi[C],2008:S359/2.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[唐伟]的文章
[张素芳]的文章
[谭海东]的文章
百度学术
百度学术中相似的文章
[唐伟]的文章
[张素芳]的文章
[谭海东]的文章
必应学术
必应学术中相似的文章
[唐伟]的文章
[张素芳]的文章
[谭海东]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。