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学科主题: 物理化学
题名: Nitric Oxide and Hydrogen Peroxide Signaling in Brassica napus Resistance to Sclerotinia sclerotiorum Induced
作者: Yin H(尹恒) ;  Zhao XM(赵小明) ;  Du YG(杜昱光)
会议名称: 2008 Annual Meeting of the Society for Glycobiology
会议日期: 2008-11-12
出版日期: 2008-11-12
会议地点: 美国
通讯作者: 杜昱光
部门归属: 十八室
主办者: glycobiology
摘要: Oligochitosan was applied by spraying it on Brassica napus leaves for control of Sclerotinia sclerotiorum(SS). The best inducing resistance concentration of oligochitosan was 50μg mL-1 and the best pretreated time was 3 days before inoculation with SS. The production of H2O2 and NO in epidermal cells induced by oligochitosan was investigated by epidermal strip bioassay and LSCM, using cell permeable fluorophore diaminofluorescein diacetate (DAF-2D) and 2’,7’- dichlorofluorescin diacetate (H2DCF-DA), respectively. Epidermal Brassica napus cells treated with oligochitosan resulted in a strong increase of intracellular NO and H2O2. Oligochitosan and NO donor sodium nitroprusside (SNP) induced the defense reaction against SS, and increased lipoxygenase (LOX) activity. mRNA levels of PDF1.2 were also induced by oligochitosan and SNP. Co-treatment of Brassica napus with oligochitosan and NO scavenger CPTIO blocked the inducing resistance. These results indicated that the defense response induced by oligochitosan was connected with NO pathway.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113210
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Yin H,Zhao XM,Du YG. Nitric Oxide and Hydrogen Peroxide Signaling in Brassica napus Resistance to Sclerotinia sclerotiorum Induced[C]. 见:2008 Annual Meeting of the Society for Glycobiology. 美国. 2008-11-12.
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