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学科主题: 物理化学
题名: Isolation and characterization of an oilseed rape SKP1 gene BnSKP1 involved on defense in Brassica napus
作者: Yin H(尹恒) ;  Li SG(李曙光) ;  Zhao XM(赵小明) ;  Bai XF(白雪芳) ;  Du YG(杜昱光)
会议名称: The 13th International Biotechnology Symposium
会议日期: 2008-10-12
出版日期: 2008-10-12
会议地点: 中国
通讯作者: 杜昱光
部门归属: 十八室
主办者: 中国科学院 中国工程院 中华人民共和国教育部 中华人民共和国科学技术部 中华人民共和国国家发展改革委员会
摘要: Oligochitosan prepared by enzymatic hydrolysis of chitosan is a potential plant defense elicitor (Zhang et al., 1999), but how the oligochitosan induces the resistance of plants to pathogens is still unclear. So we try to explore the signal transduction pathways of oligochitosan inducing defense (Wang et al., 2008). We isolated a new cDNA coding for S-phase kinase-associated protein 1 (SKP1), designated BnSKP1 (Genebank: DQ206627), from oilseed (Brassica napus L.) based on our former microarray data by RACE technology (Yin et al., 2006). BnSKP1 encodes a 160-amino-acid protein with high sequence similarity to previously reported plant SKP1 genes such as AtASK1. Bioinformatic analysis showed BnSKP1 contains the conserved domain of SKP1. By using semi-quantitative RT-PCR techniquewe found that BnMPK4 has a constitutive expression in seedling leaves and is further up-regulated upon treatment with same plant hormone. These results suggest a role for BnSKP1 in oligochitosan induced and plant defense signalling pathway.
语种: 中文
Citation statistics: 
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113222
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Yin H,Li SG,Zhao XM,et al. Isolation and characterization of an oilseed rape SKP1 gene BnSKP1 involved on defense in Brassica napus[C]. 见:The 13th International Biotechnology Symposium. 中国. 2008-10-12.
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