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学科主题物理化学
Isolation and characterization of an oilseed rape SKP1 gene BnSKP1 involved on defense in Brassica napus
Yin H(尹恒); Li SG(李曙光); Zhao XM(赵小明); Bai XF(白雪芳); Du YG(杜昱光)
会议名称The 13th International Biotechnology Symposium
会议日期2008-10-12
2008-10-12
会议地点中国
页码S227/2
部门归属十八室
主办者中国科学院 中国工程院 中华人民共和国教育部 中华人民共和国科学技术部 中华人民共和国国家发展改革委员会
英文摘要Oligochitosan prepared by enzymatic hydrolysis of chitosan is a potential plant defense elicitor (Zhang et al., 1999), but how the oligochitosan induces the resistance of plants to pathogens is still unclear. So we try to explore the signal transduction pathways of oligochitosan inducing defense (Wang et al., 2008). We isolated a new cDNA coding for S-phase kinase-associated protein 1 (SKP1), designated BnSKP1 (Genebank: DQ206627), from oilseed (Brassica napus L.) based on our former microarray data by RACE technology (Yin et al., 2006). BnSKP1 encodes a 160-amino-acid protein with high sequence similarity to previously reported plant SKP1 genes such as AtASK1. Bioinformatic analysis showed BnSKP1 contains the conserved domain of SKP1. By using semi-quantitative RT-PCR techniquewe found that BnMPK4 has a constitutive expression in seedling leaves and is further up-regulated upon treatment with same plant hormone. These results suggest a role for BnSKP1 in oligochitosan induced and plant defense signalling pathway.
语种中文
WOS记录号WOS:000208979401034
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/113222
专题中国科学院大连化学物理研究所
通讯作者Du YG(杜昱光)
推荐引用方式
GB/T 7714
Yin H,Li SG,Zhao XM,et al. Isolation and characterization of an oilseed rape SKP1 gene BnSKP1 involved on defense in Brassica napus[C],2008:S227/2.
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