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学科主题: 物理化学
题名: Determination of major secondarymetabolites in tobacco after oligochitosan treatment by HPLC with coumetric array detection
作者: Wang WX(王文霞) ;  Li SG(李曙光) ;  Zhao XM(赵小明) ;  Du YG(杜昱光) ;  Lin BC(林炳承)
会议名称: The 13th International Biotechnology Symposium
会议日期: 2008-10-12
出版日期: 2008-10-12
会议地点: 中国
通讯作者: 杜昱光
部门归属: 十八室
主办者: 中国科学院 中国工程院 中华人民共和国教育部 中华人民共和国科学技术部 中华人民共和国国家发展改革委员会
摘要: Chitin is a natural polysaccharide, which consists of a copolymer of N-acetyl-d-glucosamine and d-glucosamine residues, linked by -1,4 glycosidic bonds. Chitosan is the deacetylated form of chitin,the major component of exoskeletons of insects and crustacea, and can also be found in the cell walls of some fungi. Oligochitosan is the fragments of chitosan, prepared by enzymatic hydrolysis,with higher elicitor activity and water solubility than chitosan, applied as an effective environmentally safe means for plant disease control (Kim and Rajapakse, 2005). In the present work, A HPLC procedure utilizing reversed-phase chromatography coupled with a coulometric array detection system was developed for the characterization of severalmajor secondarymetabolites in tobacco (Schmelz et al., 2003). In a single step, leaves are extracted in aqueous acidifiedmethanol, and the extractedcompounds are separated by reversed-phase HPLC with an acidic water/acetonitrile gradient in 65 min. The utility of the method in quantifying changes in the secondary metabolites of tobacco after oligochitosan treatment, a treatment known to elicit resistance to herbivores in nature, is illustrated (Keinanen et al., 2001). Oligochitosan treatmentelicitedincreases insomesecondarymetabolites (chlorogenic acid, p-coumaric acid and nicotine increased 1.5-, 4.6-, and 2.4-fold, respectively) but left others, caffeic acid and scopoletin, undetected in both control and treat group, and rutin, changed with no significance. Such broad-based analytical screens will help characterize environmental changes in secondary metabolite profiles and illustrate mechanism of oligochitosan-induced resistance.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113230
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Wang WX,Li SG,Zhao XM,et al. Determination of major secondarymetabolites in tobacco after oligochitosan treatment by HPLC with coumetric array detection[C]. 见:The 13th International Biotechnology Symposium. 中国. 2008-10-12.
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