中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 会议论文
学科主题: 物理化学
题名: Chitin oligosaccharides production fromenzymatic hydrolyzing crab shells
作者: Wu YY(吴媛媛) ;  Lv Y(吕杨) ;  Bai XF(白雪芳) ;  Du YG(杜昱光)
会议名称: The 13th International Biotechnology Symposium
会议日期: 2008-10-12
出版日期: 2008-10-12
会议地点: 中国
通讯作者: 杜昱光
部门归属: 十八室
主办者: 中国科学院 中国工程院 中华人民共和国教育部 中华人民共和国科学技术部 中华人民共和国国家发展改革委员会
摘要: In order to find an environment friendly way of producing chitin oligosaccharides from pretreated crab shells, we hydrolyze emulsified pretreated crab shells with chitinase whose gene is from Aspergillus fumigatus YJ-407 and cloned and expressed in E. coli (Xiaet al., 2001; Anonymous, 2008). After optimizing chitinase enzymatichydrolysis conditions using Orthogonal Experimental Design, we conduct the hydrolysis experiment under the conditions which is adding 5 g chitinase to every 0.022 g emulsified crab shells and keeping this 2ml solution in 50 ◦C and pH 5.0 environment. After 75 min reaction, we analyze the hydrolyzed products by HPAECPAD (Anonymous, 2008) and TLC (Anonymous, 2008). The results showed that crab shells without fierce acid and alkali pretreatment can be readily hydrolyzed by this chitinase. There are mainly three kinds of chitin oligosaccharides in the hydrolyzed solution. They are N-acetyl-glucosamine, chitobiose and chitotriose, and chitobiose is the most abundant product. The production efficiencies are separately 7.21%, 1.15%, and 0.29% for chitobiose, N-acetyl-glucosamine and chitotriose. The result can be used as a base for the more valuable development about the trash crab shells.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113242
Appears in Collections:中国科学院大连化学物理研究所_会议论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Wu YY,Lv Y,Bai XF,et al. Chitin oligosaccharides production fromenzymatic hydrolyzing crab shells[C]. 见:The 13th International Biotechnology Symposium. 中国. 2008-10-12.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [吴媛媛]'s Articles
 [吕杨]'s Articles
 [白雪芳]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [吴媛媛]‘s Articles
 [吕杨]‘s Articles
 [白雪芳]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace