DICP OpenIR
学科主题物理化学
Protein fractioned by Microscale Solution Isoelectric Focusing and Identification by μHPLC-MS/MS
Tao DY(陶定银); Duan JC(段继诚); Liang Z(梁振); Zhang WB(张维冰); Zhang LH(张丽华); Zhang YK(张玉奎)
会议名称7th Annual World Congress HUPO2008
会议日期2008-8-16
2008-08-16
会议地点Netherlands
页码无/2
部门归属十八室
主办者HUPO Council
英文摘要Comprehensive analysis of whole proteomes is an extraordinary challenge because of the complexity and wide dynamic range of proteins. The success of proteome analysis is highly dependent on the quality of sample prefractionation prior to the identification by MS/MS. In our recent work, preparative microscale solution isoelectric focusing (PMSIEF) was used to achieve narrow-band fractionation of proteins from Escherichia coli (E. coli). Proteins in various pH related fractions were further separated using coupled long reversed phase high-performance liquid chromatography (RP-HPLC) columns and identified by MS/MS.
语种中文
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/113382
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
推荐引用方式
GB/T 7714
Tao DY,Duan JC,Liang Z,et al. Protein fractioned by Microscale Solution Isoelectric Focusing and Identification by μHPLC-MS/MS[C],2008:无/2.
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