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学科主题: 物理化学
题名: Protein fractioned by Microscale Solution Isoelectric Focusing and Identification by μHPLC-MS/MS
作者: Tao DY(陶定银) ;  Duan JC(段继诚) ;  Liang Z(梁振) ;  Zhang WB(张维冰) ;  Zhang LH(张丽华) ;  Zhang YK(张玉奎)
会议名称: 7th Annual World Congress HUPO2008
会议日期: 2008-8-16
出版日期: 2008-08-16
会议地点: Netherlands
通讯作者: 张丽华
部门归属: 十八室
主办者: HUPO Council
摘要: Comprehensive analysis of whole proteomes is an extraordinary challenge because of the complexity and wide dynamic range of proteins. The success of proteome analysis is highly dependent on the quality of sample prefractionation prior to the identification by MS/MS. In our recent work, preparative microscale solution isoelectric focusing (PMSIEF) was used to achieve narrow-band fractionation of proteins from Escherichia coli (E. coli). Proteins in various pH related fractions were further separated using coupled long reversed phase high-performance liquid chromatography (RP-HPLC) columns and identified by MS/MS.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113382
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Tao DY,Duan JC,Liang Z,et al. Protein fractioned by Microscale Solution Isoelectric Focusing and Identification by μHPLC-MS/MS[C]. 见:7th Annual World Congress HUPO2008. Netherlands. 2008-8-16.
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