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学科主题: 物理化学
题名: A Facile Buffer Exchange Interface for On-line Hyphenation with Trypsin Microreactor
作者: Sun LL(孙良亮) ;  Qiao XQ(乔晓强) ;  Tao DY(陶定银) ;  Liang Z(梁振) ;  Zhang WB(张维冰) ;  Zhang LH(张丽华) ;  Zhang YK(张玉奎)
会议名称: 7th Annual World Congress HUPO2008
会议日期: 2008-8-16
出版日期: 2008-08-16
会议地点: Netherlands
通讯作者: 张丽华
部门归属: 十八室
主办者: HUPO Council
摘要: For shotgun method, protein digestion is one of the most important steps, which is usually performed in solution, but recently more focus is put on the trypsin microreactor because of the high digestion efficiency. However, in most cases, the pH and organic reagent concentration of protein solutions, especially for eluants from HPLC separation, are not compatible with immobilized trypsin. Therefore, it is indispensable to develop on-line buffer exchange interfaces (1). In our work, a novel online buffer exchange interface based on microdialysis was developed, and successfully applied into the adjustment of pH and acetonitrile concentration successfully within 30 seconds.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113384
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Sun LL,Qiao XQ,Tao DY,et al. A Facile Buffer Exchange Interface for On-line Hyphenation with Trypsin Microreactor[C]. 见:7th Annual World Congress HUPO2008. Netherlands. 2008-8-16.
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