Subject Area物理化学
A Facile Buffer Exchange Interface for On-line Hyphenation with Trypsin Microreactor
Sun LL(孙良亮); Qiao XQ(乔晓强); Tao DY(陶定银); Liang Z(梁振); Zhang WB(张维冰); Zhang LH(张丽华); Zhang YK(张玉奎)
Conference Name7th Annual World Congress HUPO2008
Conference Date2008-8-16
Conference PlaceNetherlands
Funding OrganizationHUPO Council
AbstractFor shotgun method, protein digestion is one of the most important steps, which is usually performed in solution, but recently more focus is put on the trypsin microreactor because of the high digestion efficiency. However, in most cases, the pH and organic reagent concentration of protein solutions, especially for eluants from HPLC separation, are not compatible with immobilized trypsin. Therefore, it is indispensable to develop on-line buffer exchange interfaces (1). In our work, a novel online buffer exchange interface based on microdialysis was developed, and successfully applied into the adjustment of pH and acetonitrile concentration successfully within 30 seconds.
Document Type会议论文
Corresponding AuthorZhang LH(张丽华)
Recommended Citation
GB/T 7714
Sun LL,Qiao XQ,Tao DY,et al. A Facile Buffer Exchange Interface for On-line Hyphenation with Trypsin Microreactor[C],2008:无/2.
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