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学科主题物理化学
A Facile Buffer Exchange Interface for On-line Hyphenation with Trypsin Microreactor
Sun LL(孙良亮); Qiao XQ(乔晓强); Tao DY(陶定银); Liang Z(梁振); Zhang WB(张维冰); Zhang LH(张丽华); Zhang YK(张玉奎)
会议名称7th Annual World Congress HUPO2008
会议日期2008-8-16
2008-08-16
会议地点Netherlands
页码无/2
部门归属十八室
主办者HUPO Council
英文摘要For shotgun method, protein digestion is one of the most important steps, which is usually performed in solution, but recently more focus is put on the trypsin microreactor because of the high digestion efficiency. However, in most cases, the pH and organic reagent concentration of protein solutions, especially for eluants from HPLC separation, are not compatible with immobilized trypsin. Therefore, it is indispensable to develop on-line buffer exchange interfaces (1). In our work, a novel online buffer exchange interface based on microdialysis was developed, and successfully applied into the adjustment of pH and acetonitrile concentration successfully within 30 seconds.
语种中文
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/113384
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
推荐引用方式
GB/T 7714
Sun LL,Qiao XQ,Tao DY,et al. A Facile Buffer Exchange Interface for On-line Hyphenation with Trypsin Microreactor[C],2008:无/2.
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