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学科主题: 物理化学
题名: Targeted chitosan/siRNA nanoparticles for effective treatment of cancer
作者: Yang Y(扬艳) ;  Liu XD(刘袖洞) ;  Ma XJ(马小军)
会议名称: Roche Marco Polo Symposium 2008
会议日期: 2008-12-3
出版日期: 2008-12-03
会议地点: 中国
通讯作者: 马小军
部门归属: 十八室
主办者: Roche Shanghai
摘要: RNA interference (RNAi) using small interfering RNA (siRNA) might provide a way to silence disease-associated genes. Although potent sequence-selective gene silencing by siRNA promises the ultimate level of specificity, naked siRNA therapeutics is hindered by low blood stability, non-specific immune stimulation, and poor intracellular uptake. To address these problems, nano-sized carriers, especially those ligand-targeted, sterically stabilized nanoparticles should be an ideal candidate for the effective protection and delivery of siRNA. In our study, biocompatible and biodegradable chitosan (CTS) has been chosen as carrier material for siRNA. Cell adhesive peptide GRGDY has been grafted to CTS by photosensitive crosslinker to target integrin receptor over-expressed on the surface of tumor cells. FTIR、MALDI-MS and 1H-NMR spectrum have proven the successful synthesis of ligand modified chitosan (mCTS). Moreover, CTS (mCTS)/siRNA nanoparticles was formulated with the size of 200-300 nm and the positive surface charge of ~20 mV. PAGE showed the complex stability of nanoparticles and efficiency of protecting siRNA from RNase degradation. The nanoparticles can transfer and protect entrapped siRNA into mouse melanoma cells in vitro, without apparent critical cytotoxicity. The further evaluations of targeted CTS-siRNA nanoparticles regarding to the silencing efficiency upon tumor related gene are undergoing.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113418
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Yang Y,Liu XD,Ma XJ. Targeted chitosan/siRNA nanoparticles for effective treatment of cancer[C]. 见:Roche Marco Polo Symposium 2008. 中国. 2008-12-3.
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