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学科主题: 物理化学
题名: Control biodegradation of chitosan -alginate scaffolds
作者: Li XX(李晓霞) ;  Xie HG(谢红国) ;  Xie WY(谢威扬) ;  Ma XJ(马小军)
会议名称: Thidteenth intertional biotechnology symposium and exhibition
会议日期: 2008-10-12
出版日期: 2008-10-12
会议地点: 中国
通讯作者: 马小军
ISSN: 0168-1656
部门归属: 十八室
主办者: 中国科学院
摘要: Recently, much attention has been given to natural polymeric scaffolds for tissue engineering and drug deivery systems [1]. Chitosan has been widely used in this area because of its excellent mechanical properties, biocompatibility and biodegradation [2]. The goals of this work were to investigate the biodegradable behaviors of chitosan-alginate scaffolds with different ratios of chitosan and alginate, which were prepared by freeze-drying methods [3]. FTIR spectrums suggested the formation of the chitosan–alginate complex in the scaffolds as a result of the ionic interaction between the opposite charged polymers. The degradation experiments were carried out in NaCl solution containing 0.5 mg/mL of lysozyme at 37°C, and the biodegradation rates were tested by measuring the change in reducing sugar, sample weight and lysozyme adsorption over time. It appears that chitosan scaffold with low degree of deacetylation was susceptible to lysozyme [4], as can be seen in the Fig that the amount of reducing sugar released from chitosan (CS60) increased fast with 1703 μmol/g at 56 days; however, after mixing with alginate, the biodegradation rate decreased, maybe due to a reduced incidence of collision between chitosan and lysozyme. The higher the ratio of alginate, the lower the sugar released. The degradation rate of A1C2 (Alginate:Chitosan=1:2) by hydrolysis alone (without lysozyme) was very low as shown by C-A1C2 in the Fig. The weight remaining of the scaffolds were not consistent with their degradation rates because of lysozyme adsorption, which increased with the ratio of alginate. For example, the degradation rate was slow for A2C1, but the amount of adsorbed lysozyme kept at about 290mg/g after 3 days, so its weight increased to 117%. The weights of A1C2, A1C4 and CS60 showed some decrease as less enzyme adsorption and faster degradation rate. C-A1C2 kept its weight unchanged in NaCl solution. In summary, the desirable degradation rate of chitosan-alginate scaffolds can be achieved through adjusting the ratio of chitosan and alginate to suit the need of biodegradable medical engineering.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113422
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Li XX,Xie HG,Xie WY,et al. Control biodegradation of chitosan -alginate scaffolds[C]. 见:Thidteenth intertional biotechnology symposium and exhibition. 中国. 2008-10-12.
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