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学科主题: 物理化学
题名: Design a chemically defined/medically approved medium for microencapsulated cells
作者: Lv GJ(吕国军) ;  Ma XJ(马小军)
会议名称: 13th International Biotechnology Symposium & Exhibition
会议日期: 2008-10-12
出版日期: 2008-10-12
会议地点: 中国
通讯作者: 马小军
部门归属: 十八室
主办者: 中科院大连化物所
摘要: Design a chemically defined/medically approved medium for microencapsulated cells Purpose of study: Implantation of microencapsulation of recombinant cells has great potential for gene therapy where therapeutic protein can be sustained and long-term delivered by microencapsulated cells [1]. However, once cells are encapsulated in microcapsules, they need to be cultured or preserved in serum free medium or chemical defined additives that are medically approved to maintain the cell viability before implantation. Based on the metabolic characteristics of microencapsulated cells, the purpose of this study is to develop a simple, low protein, microcapsules suited and medically approved chemically defined medium for microencapsulated cell culture. Methods: Firstly, the metabolic characteristics of amino acid were assayed for non-encapsulated cells and encapsulated cells. According to this result, the optimum amino acid supplements were adjusted to the base medium. Secondly, orthogonal test design was applied to select a suitable chemically defined medium for microencapsulated cells. Finally, the effects of the optimized medium on the microencapsulated cells viability, therapeutic protein production and long-term in vitro stability of APA microcapsules were evaluated. Results and Conclusions: The results show that the optimized medium significantly enhances cell survival and, particularly, endostatin secretion of encapsulated CHO cells over a time period of 8 weeks. Furthermore, long-term in vitro stability of Alginate-poly-lysine-alginate (APA) microcapsules underwent no changes, which indicated that this optimized medium might be feasible to maintain high viability of microencapsulated cell for short periods of time before implantation.
语种: 中文
Citation statistics: 
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113434
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Lv GJ,Ma XJ. Design a chemically defined/medically approved medium for microencapsulated cells[C]. 见:13th International Biotechnology Symposium & Exhibition. 中国. 2008-10-12.
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