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学科主题: 物理化学
题名: Solubilization and Digestion of Integral Membrane Proteins in Ionic Liquid Solvent System
作者: Sun LL(孙良亮) ;  Han B(韩彬) ;  Tao DY(陶定银) ;  Liang Z(梁振) ;  Zhang LH(张丽华) ;  Zhang YK(张玉奎)
会议名称: HUPO 8th Annual World Congress
会议日期: 2009-9-26
出版日期: 2009-09-26
会议地点: 加拿大
通讯作者: 张丽华
部门归属: 十八室
主办者: York University
摘要: Integral membrane proteins (IMPs) play unique roles in mediating cellular processes. Up till now, four different kinds of buffers, including chaotropes, detergents, organic solvents and organic acids (1), have been applied to improve the solubility of membrane proteins. In this work, ionic liquid (IL), 1-butyl-3-methyl imidazolium tetrafluoroborate, was used to dissolve membrane proteins extracted from Rat cerebellum, and more proteins were identified by μRPLC-ESI-MS/MS comparing to those dissolved by SDS and methanol.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113594
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Sun LL,Han B,Tao DY,et al. Solubilization and Digestion of Integral Membrane Proteins in Ionic Liquid Solvent System[C]. 见:HUPO 8th Annual World Congress. 加拿大. 2009-9-26.
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