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学科主题: 物理化学
题名: Protein Equalizer Based on scFv Displaying M13 Phage Library for Serum Proteome Analysis
作者: Zhao P(赵鹏) ;  Zhang LH(张丽华) ;  Liang Z(梁振) ;  Zhang YK(张玉奎)
会议名称: HUPO 8th Annual World Congress
会议日期: 2009-9-26
出版日期: 2009-09-26
会议地点: 加拿大
通讯作者: 张丽华
ISSN: 1542-6416
部门归属: 十八室
主办者: York University
摘要: The decrease of proteome complexity by removing high abundance proteins is essential to discover low abundance ones with important biological functions. Although antibody columns are proven effective to deplete several kinds of high abundance proteins (1), it is impossible to remove unknown high abundance proteins. Righetti proposed the technique of protein equalizer by immobilizing combinatorial peptide ligands on silica, by which proteins were bound to beads according to the specific interaction with ligands instead of the amount (2). In our recent work, a novel protein equalizer based on scFv displaying M13 phage library was developed with monolithic cryogel as the matrix, by which the identified protein number from human serum was nearly increased by a factor of 1.85.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113596
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Zhao P,Zhang LH,Liang Z,et al. Protein Equalizer Based on scFv Displaying M13 Phage Library for Serum Proteome Analysis[C]. 见:HUPO 8th Annual World Congress. 加拿大. 2009-9-26.
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