DICP OpenIR
学科主题物理化学
Protein Equalizer Based on scFv Displaying M13 Phage Library for Serum Proteome Analysis
Zhao P(赵鹏); Zhang LH(张丽华); Liang Z(梁振); Zhang YK(张玉奎)
会议名称HUPO 8th Annual World Congress
会议日期2009-9-26
2009-09-26
会议地点加拿大
页码126/1
ISSN1542-6416
部门归属十八室
主办者York University
英文摘要The decrease of proteome complexity by removing high abundance proteins is essential to discover low abundance ones with important biological functions. Although antibody columns are proven effective to deplete several kinds of high abundance proteins (1), it is impossible to remove unknown high abundance proteins. Righetti proposed the technique of protein equalizer by immobilizing combinatorial peptide ligands on silica, by which proteins were bound to beads according to the specific interaction with ligands instead of the amount (2). In our recent work, a novel protein equalizer based on scFv displaying M13 phage library was developed with monolithic cryogel as the matrix, by which the identified protein number from human serum was nearly increased by a factor of 1.85.
语种中文
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/113596
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
推荐引用方式
GB/T 7714
Zhao P,Zhang LH,Liang Z,et al. Protein Equalizer Based on scFv Displaying M13 Phage Library for Serum Proteome Analysis[C],2009:126/1.
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