中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 会议论文
学科主题: 物理化学
题名: High sensitive protein detection with in-situ fluorescence derivatization
作者: Qiao XQ(乔晓强) ;  Sun LL(孙良亮) ;  Liang Z(梁振) ;  Zhang LH(张丽华) ;  Zhang YK(张玉奎)
会议名称: The 24th MSB 2009
会议日期: 2009-10-19
出版日期: 2009-10-19
会议地点: 中国
通讯作者: 张丽华
部门归属: 十八室
主办者: 大连化物所
摘要: In the post-genome sequencing era, high sensitive protein analysis has been paid much attention. Although native fluorescence emitted by aromatic amino acid residues is an effective tool for proteins detection, not all proteins could generate intensive native fluorescence signal. Thus, various fluorescence reagents have been synthesized and applied for protein derivatization. However, the derivatization of low abundance proteins is still challenging. Taking the traditional fluorescence reagent, FITC, as an example, the limit of detection for proteins is similar to that of UV detection. In current work, a novel in-situ fluorescence derivatization method based on hollow fiber membrane interface was developed to achieve high efficient derivatization and high sensitive detection of proteins, by which proteins were firstly concentrated in hollow fiber membrane, and then in situ derivatized by submerging the membrane into fluorescence buffer. With such a method, the high sensitive detection of proteins with pM concentration were achieved by HPLC with a fluorescence detector. Furthermore, the derivatization of a fraction of E. coli whole cell lysate were achieved, and high sensitive detection was obtained compared to that obtained by UV detection and conventional in-solution fluorescence derivatization.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113614
Appears in Collections:中国科学院大连化学物理研究所_会议论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Qiao XQ,Sun LL,Liang Z,et al. High sensitive protein detection with in-situ fluorescence derivatization[C]. 见:The 24th MSB 2009. 中国. 2009-10-19.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [乔晓强]'s Articles
 [孙良亮]'s Articles
 [梁振]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [乔晓强]‘s Articles
 [孙良亮]‘s Articles
 [梁振]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace