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学科主题: 物理化学
题名: Zirconia-based mesoporous aerogel for high throughput phosphoproteome enrichment
作者: Zhang LY(张丽媛) ;  Wang H(王晖) ;  Liang Z(梁振) ;  Zhang LH(张丽华) ;  Zhang YK(张玉奎)
会议名称: The 24th MSB 2009
会议日期: 2009-10-19
出版日期: 2009-10-19
会议地点: 中国
通讯作者: 张丽华
部门归属: 十八室
主办者: 大连化物所
摘要: As one of the most important reversible modifications in nature, protein phosphorylation plays an important role in cell division, signal transduction, enzymatic activity and so on. However, the low abundance of phosphopeptides and the signal suppression by nonphosphopeptides render the comprehensive characterization of phosphoproteome still a challenge. Therefore, several strategies have been developed for the selective enrichment of phosphopeptides prior to MS analysis. Immobilized metal affinity chromatography (IMAC)has been widely employed for the enrichment of phosphopeptides. However, the non-specific binding of acidic non-phosphopeptides is still hard to overcome. Recently, metal oxide nanoparticles, such as zirconia (ZrO2) and titanium dioxide (TiO2), were employed in both on-line and off-line LC-MS analysis, and improved selectivity enrichment for phosphopeptides was achieved compared to that obtained by conventional IMAC materials. However, the relative low specific surface area of commercial metal oxide nanoparticles limits the enrichment capacity. In our work, zirconia-based mesoporous aerogel with high specific surface area was prepared by simple sol-gel process, followed by supercritical fluid drying and calcination. The specific surface area of such a novel material is 416.0 m2/g, nearly 10 folds higher than those of commercial zirconia nanoparticles. With α-casein digests as an sample, high selectivity and improved enrichment capacity of phosphopeptides were achieved, indicating the potential of zirconia-based mesoporous aerogel in phosphoproteome research.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113616
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Zhang LY,Wang H,Liang Z,et al. Zirconia-based mesoporous aerogel for high throughput phosphoproteome enrichment[C]. 见:The 24th MSB 2009. 中国. 2009-10-19.
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