DICP OpenIR
学科主题物理化学
Protein separation by CZE with ionic liquid as buffer additives
Han B(韩彬); Cong YZ(丛永正); Zhang LH(张丽华); Qu F(屈锋); Deng YL(邓玉林); Zhang YK(张玉奎)
会议名称The 24th MSB 2009
会议日期2009-10-19
2009-10-19
会议地点中国
页码245/1
部门归属十八室
主办者大连化物所
英文摘要The development of novel analytical techniques and methods is very important for proteome analysis. Capillary zone electrophoresis (CZE), with the advantages of high efficiency, high speed, low consumption of samples and solvents, is quite popular for biomolecules separation. However, to avoid the non-specific absorption of proteins on the inner wall of capillaries, various methods have been proposed. Recently, ionic liquids were used as dynamic coating and running buffer in CZE, by which the non-specific adsorption of samples were effectively suppressed, and the separation was improved. However, the low buffer ability and poor stability of ionic liquids make them not suitable to be used as the only component of CZE buffer. In our recent work, ionic liquids were used as additives in boric buffer, and applied for protein separation by CZE with dynamic coating of imidazolium cations. By such a method, a mixture of cytochrome C, myoglobin, carbonic anhydrase were separated with good resolution and high efficiency. Furthermore, compared to the separation with only ionic liquids as buffer, not only good reproducibility was obtained, but also proteins could be enriched by sample stacking effect.
语种中文
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/113620
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
推荐引用方式
GB/T 7714
Han B,Cong YZ,Zhang LH,et al. Protein separation by CZE with ionic liquid as buffer additives[C],2009:245/1.
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