Subject Area物理化学
Preparation of albumin imprinted nanoparticles by coprecipitation method
Liu JX(刘晋湘); Deng QL(邓启良); Zhang LH(张丽华); Liang Z(梁振); Zhang YK(张玉奎)
Conference NameThe 24th MSB 2009
Conference Date2009-10-19
Conference Place中国
Funding Organization大连化物所
AbstractMolecular imprinting is a promising technique for the preparation of tailor-made synthetic materials with selectivity for target molecules1, 2. Although various molecular imprinting polymers (MIPs) have been successfully prepared with small molecules imprinted, few literatures were reported on MIPs for the recognition of biological macromolecules, due to large molecular size, physiochemical complexity, conformational flexibility and poor stability and solubility in non-polar solvent. To overcome the disadvantage of poor mass transfer of traditional in bulk imprinting materials, surface imprinting technique has been proposed3,4. Here, a new surface imprinting method was proposed to prepare MIPs with porcine serum albumin (PSA) as the template by coprecipitation. Methacrylic acid and ethylene glycol dimethacrylate were respectively used as functional monomers and cross-linkers. Salt crystal and template protein were coprecipitated in organic solvent, and polymerization was performed around salt-protein complex. After removing template and salt by water, PSA imprinted nanopaticles were obtained. SEM images present that MIP particles are uniform nanospheres with diameter of 400-500 nm. The binding experiment shows that MIPs exhibit high selectivity for PSA in a competitive environment, and the imprinting factor of MIPs was about 50. In addition, the easy removal of porogens and template proteins by water makes the approach a new way to synthesize MIPs with proteins as templates.
Document Type会议论文
Corresponding AuthorZhang LH(张丽华)
Recommended Citation
GB/T 7714
Liu JX,Deng QL,Zhang LH,et al. Preparation of albumin imprinted nanoparticles by coprecipitation method[C],2009:247/1.
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