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学科主题: 物理化学
题名: Preparation of Monolithic Immobilized pH Gradient Materials in Capillaries and Microchannels by Photopolymerization and Photografting for Isoelectric Focusing of Proteins
作者: Liang Y(梁玉) ;  Cong YZ(丛永正) ;  Zhang LH(张丽华) ;  Zhang YK(张玉奎)
会议名称: 34th international Symposium on High-Performance liquid Phase Separations and Related Techniques
会议日期: 2009-6-28
出版日期: 2009-06-28
会议地点: 德国
通讯作者: 张丽华
部门归属: 十八室
主办者: Arbeitskreis Separation Science German Chemical Society (GDCh)
摘要: Capillary isoelectric focusing has been regarded as a powerful tool for protein analysis with advantages of high resolution and high enrichment capacitu.Recently, with the development of u-TAS techniques,CIEF has been adapted to microchips, and shown the potentials of rapid protein analysis and straightforward integration capacity added in running buffer to establish stable pH gradient during focusing.However, mobile CAs in buffer might lead to increased current and joule heat during focusing. In additon, they might affect not only sample detection by UV at low wavelength and MS,but also further multidimensional separation.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113648
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Liang Y,Cong YZ,Zhang LH,et al. Preparation of Monolithic Immobilized pH Gradient Materials in Capillaries and Microchannels by Photopolymerization and Photografting for Isoelectric Focusing of Proteins[C]. 见:34th international Symposium on High-Performance liquid Phase Separations and Related Techniques. 德国. 2009-6-28.
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