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学科主题: 物理化学
题名: Spectroscopic Evidence of Human Telomeric G-quadruplex
作者: Zhou J(周俊) ;  Feng ZC(冯兆池) ;  Li C(李灿)
会议名称: 第十三届亚洲化学会
会议日期: 2009-9-13
出版日期: 2009-09-13
会议地点: China
通讯作者: 李灿
部门归属: 五室
主办者: Chinese Chemical Society
摘要: The structural interconversion between the G-quadruplex and duplex in vivo is an important subject because it is believed that the formation of G-quadruplex structure depends on the denaturation of the duplex [1]. In the present study, we used human telomeric DNA duplex composed of GGG(TTAGGG)3/ CCC(TAACCC)3 as a model system to investigate its properties under near physiological conditions by spectroscopic methods. Circular dichroism and fluorescence spectra demonstrated that G-quadruplex structure can be formed from duplex at near physiological pH (pH 7.4), salt concentration (150 mM K+), and temperature (37 oC) in the presence of molecular crowding agent PEG (40 %), whereas the G-quadruplex structure can not be formed at 25 oC in buffer containing 150 mM K+ in the presence of PEG. This work suggests that human telomeric G-quadruplex structure may be potentially formed from Watson-Crick duplex in vivo.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113816
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Zhou J,Feng ZC,Li C. Spectroscopic Evidence of Human Telomeric G-quadruplex[C]. 见:第十三届亚洲化学会. China. 2009-9-13.
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