DICP OpenIR
Subject Area物理化学
Spectroscopic Evidence of Human Telomeric G-quadruplex
Zhou J(周俊); Feng ZC(冯兆池); Li C(李灿)
Conference Name第十三届亚洲化学会
Conference Date2009-9-13
2009-09-13
Conference PlaceChina
Pages77/1
Department五室
Funding OrganizationChinese Chemical Society
AbstractThe structural interconversion between the G-quadruplex and duplex in vivo is an important subject because it is believed that the formation of G-quadruplex structure depends on the denaturation of the duplex [1]. In the present study, we used human telomeric DNA duplex composed of GGG(TTAGGG)3/ CCC(TAACCC)3 as a model system to investigate its properties under near physiological conditions by spectroscopic methods. Circular dichroism and fluorescence spectra demonstrated that G-quadruplex structure can be formed from duplex at near physiological pH (pH 7.4), salt concentration (150 mM K+), and temperature (37 oC) in the presence of molecular crowding agent PEG (40 %), whereas the G-quadruplex structure can not be formed at 25 oC in buffer containing 150 mM K+ in the presence of PEG. This work suggests that human telomeric G-quadruplex structure may be potentially formed from Watson-Crick duplex in vivo.
Language中文
Document Type会议论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/113816
Collection中国科学院大连化学物理研究所
Corresponding AuthorLi C(李灿)
Recommended Citation
GB/T 7714
Zhou J,Feng ZC,Li C. Spectroscopic Evidence of Human Telomeric G-quadruplex[C],2009:77/1.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[周俊]'s Articles
[冯兆池]'s Articles
[李灿]'s Articles
Baidu academic
Similar articles in Baidu academic
[周俊]'s Articles
[冯兆池]'s Articles
[李灿]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[周俊]'s Articles
[冯兆池]'s Articles
[李灿]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.