Subject Area物理化学
Spectroscopic Evidence of Human Telomeric G-quadruplex
Zhou J(周俊); Feng ZC(冯兆池); Li C(李灿)
Conference Name第十三届亚洲化学会
Conference Date2009-9-13
Conference PlaceChina
Funding OrganizationChinese Chemical Society
AbstractThe structural interconversion between the G-quadruplex and duplex in vivo is an important subject because it is believed that the formation of G-quadruplex structure depends on the denaturation of the duplex [1]. In the present study, we used human telomeric DNA duplex composed of GGG(TTAGGG)3/ CCC(TAACCC)3 as a model system to investigate its properties under near physiological conditions by spectroscopic methods. Circular dichroism and fluorescence spectra demonstrated that G-quadruplex structure can be formed from duplex at near physiological pH (pH 7.4), salt concentration (150 mM K+), and temperature (37 oC) in the presence of molecular crowding agent PEG (40 %), whereas the G-quadruplex structure can not be formed at 25 oC in buffer containing 150 mM K+ in the presence of PEG. This work suggests that human telomeric G-quadruplex structure may be potentially formed from Watson-Crick duplex in vivo.
Document Type会议论文
Corresponding AuthorLi C(李灿)
Recommended Citation
GB/T 7714
Zhou J,Feng ZC,Li C. Spectroscopic Evidence of Human Telomeric G-quadruplex[C],2009:77/1.
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