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学科主题: 物理化学
题名: Efficient Enrichment and Identification of Phosphopeptides by Cerium Oxide Using on-plate MALDI-TOF MS Analysis
作者: Sun ST(孙树涛) ;  Han GH(韩广辉) ;  Zhao L(赵梁) ;  Wu RA(吴仁安) ;  Zou HF(邹汉法)
会议名称: The 24th International Symposium on Micro-scale Bioseparation,
会议日期: 2009-10-19
出版日期: 2009-10-19
会议地点: 中国
通讯作者: 邹汉法
部门归属: 十八室
主办者: 大连化学物理研究所
摘要: An efficient and simple method for enrichment and identification of phosphopeptides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) using cerium oxide is presented. After pretreatment of tryptic digests of phosphoproteins with CeO2, nonphosphopeptides are discarded and phosphopeptides are enriched. By applying the separated CeO2 on target plate and analysis using MALDI-TOF MS, peaks of phosphopeptides and their correspondingly series of dephosphorylated peptides are observed in the mass spectra. Thus, the phosphopeptides are very easy to be identified with the mass difference, which are all 80 Da between adjacent peaks in the same serials and clear background in the spectra owing to discard of signal suppression from large amount of nonphosphopeptides. Furthermore, the phosphopeptides can be dephosphorylated completely after a further NH4OH elution. Tryptic digest products from several standard proteins are pretreated using CeO2 to demonstrate the efficiency of this method. And phosphopeptides from a very small quantity of human serum are also enriched and identified, which indicates that this method may be employed in more complex sample for further application.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113846
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Sun ST,Han GH,Zhao L,et al. Efficient Enrichment and Identification of Phosphopeptides by Cerium Oxide Using on-plate MALDI-TOF MS Analysis[C]. 见:The 24th International Symposium on Micro-scale Bioseparation,. 中国. 2009-10-19.
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