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学科主题: 物理化学
题名: A Multidimensional Chromatography Technology for Global Phosphoproteome Analysis of Human Liver
作者: Song CX(宋春霞) ;  Han GH(韩广辉) ;  Jiang XN(江新宁) ;  Yu ZY(于志远) ;  Chen R(陈锐) ;  Miao N(苗楠) ;  Ye ML(叶明亮) ;  Zou HF(邹汉法)
会议名称: The 24th International Symposium on Micro-scale Bioseparation,
会议日期: 2009-10-19
出版日期: 2009-10-19
会议地点: 中国
通讯作者: 邹汉法
部门归属: 十八室
主办者: 大连化学物理研究所
摘要: Protein phosphorylation is one of the most important post-translational modifications, which regulates essential functions such as cell division, signal transduction, and so forth. However, the analysis of protein phosphorylation faces great challenges due to its highly dynamic nature and low stoichiometry. It is necessary to reduce the complexity of the samples to identification of phosphorylation sites and quantitation of phosphopeptides. We developed and evaluated a two-dimensional separation system for phosphopeptide analysis by employing reversed-phase columns in both separation dimensions. The greatest orthogonality of 2D separation was achieved for pH 7.5 in the first and pH 2.6 in the second LC dimension. Combination of this multidimensional system with immobilized metal affinity chromatography, high-accuracy mass spectrometry instrumentation analysis, and target-decoy database search strategy, the identification of 7,419 nonredundant phosphorylation sites from 2,455 proteins from 40mg human liver at false discovery rate of 1.0% was achieved. Above results indicated that RP-RP-HPLC strategy is superior to the SCX-RP system, which is one of the most popular strategy in shotgun proteome analysis, in large-scale analysis of protein phosphorylation.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113848
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Song CX,Han GH,Jiang XN,et al. A Multidimensional Chromatography Technology for Global Phosphoproteome Analysis of Human Liver[C]. 见:The 24th International Symposium on Micro-scale Bioseparation,. 中国. 2009-10-19.
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