DICP OpenIR
学科主题物理化学
MALDI-TOF MS with a Matrix of 3, 4-diaminobenzophenone for the Analysis of Phospholipids
Miao N(苗楠); Pan CS(潘陈松); Zhou HJ(周厚江); Zou HF(邹汉法)
会议名称The 24th International Symposium on Micro-scale Bioseparation,
会议日期2009-10-19
2009-10-19
会议地点中国
页码263/1
部门归属十八室
主办者大连化学物理研究所
英文摘要Lipids and phospholipids are very important biomolecules except proteins, carbohydrates and nucleic acids[1]. Phospholipids have also been proved to be specific cancer biomarkers[2]. Therefore, there are increasing interests in lipid and phospholipids analysis. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been shown previously to be an effective tool for the analysis of lipids, especially for the lipid composition of extracts of tissues and cells[3]. The new matrix 3, 4-diaminobenzophenone (DABP) is applied in the analysis of phospholipids by MALDI-TOF MS. The optimal composition of DABP matrix solution with methanol/water/hydrochloric acid (80:20:3,v/v/v) is used in the analysis of phospholipids. The most significant advantage of DABP matrix is that it suppresses the ion adducts of alkali metal, and thus showing single signal peak of phospholipids. This is very important for the analysis of the complex biological samples. In the analysis of practical samples, we do not need any matrix additives. With DABP as a matrix, intact phospholipid ion peaks can be readily produced with clean spectrum background, low laser energy, strong peak strength and high resolution. In addition, the sample preparation method is simple and time-saving. It should be concluded that different classes of phospholipids should be analyzed with different ion detection modes. Phosphatidylcholine (PC), lysophosphatidylcholine (LPC), and sphigomyelin (SM) should be analyzed under positive ion mode; and the acidic phospholipids such as phosphatidic acid (PA) and phosphatidylserine (PS) under negative ion mode.Though the signal suppression of different phospholipids species still exist, which need sample pretreatment methods such as HPLC, TLC to separate the class of lipids initially, the DABP as a matrix in lipid analysis has great potential.
语种中文
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/113852
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法)
推荐引用方式
GB/T 7714
Miao N,Pan CS,Zhou HJ,et al. MALDI-TOF MS with a Matrix of 3, 4-diaminobenzophenone for the Analysis of Phospholipids[C],2009:263/1.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[苗楠]的文章
[潘陈松]的文章
[周厚江]的文章
百度学术
百度学术中相似的文章
[苗楠]的文章
[潘陈松]的文章
[周厚江]的文章
必应学术
必应学术中相似的文章
[苗楠]的文章
[潘陈松]的文章
[周厚江]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。