中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 会议论文
学科主题: 物理化学
题名: Profiling of N-linked Glycans from Human Serum with Oxidative Ordered Mesoporous Carbon
作者: Qin HQ(秦洪强) ;  Wu RA(吴仁安) ;  Pan CS(潘陈松) ;  Chen R(陈锐) ;  Zhao L(赵梁) ;  Zou HF(邹汉法)
会议名称: The 24th International Symposium on Micro-scale Bioseparation,
会议日期: 2009-10-19
出版日期: 2009-10-19
会议地点: 中国
通讯作者: 邹汉法
部门归属: 十八室
主办者: 大连化学物理研究所
摘要: Glycosylation is one of the most common forms of post-translational modifications on proteins, and glycoproteins account more than 50% in eukaryotic proteins. Also, glycoproteins play key roles in cell-cell recognition, cancer metastasis, the immune system, and other biological functions1. Because of complicated glycosylation sites and variable glycan structures, the study of glycoproteins is difficult, while the analysis of glycans released from glycoproteins is much easier. Of all the process, purification glycans from the mixtures is the key step. Organic solvent precipitation and chromatographic separation are two main methods now used to extract glycans from proteins. However, it can’t remove proteins absolutely from the mixtures of glycans and proteins. Chromatography, including volume exclusion and hydrophilic, can be used to enrich glycans from proteins with complex procedures. Because of special interaction between oligosacchride and carbon, graphitized was used to enrich glycans released from glycoprotein and salt elimination with proteins removal first in the recent years. So, purification of glycans from the mixtures is still complicated and time-consuming. In this paper, we use oxidative ordered mesoporous carbons (O-OMC) to enrich glycans from the mixtures directly and detected by MALDI-TOF MS, without the removal of proteins firstly. With mild surface oxide, the resulting hydrophilic surface and mesoporous of the particles2 can reduces the absorption of proteins,while the special interaction between carbon and oligosaccharides can lead to selective enrichment of glycans. We select ovalbumin as standard glycoprotein and analyze all of the 24 released glycans, and 15 glycans are successfully detected. 38 of N-linked glycans distribution in human serum and deference between normal and liver cancer are also evaluated. With convenient sample handling and high through detection, this method could be used in disease diagnosis, such as early cancer confirmation. Key words: Glycans, O-OMC, Size exclusion, MALDI-TOF/MS, Human serum.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113854
Appears in Collections:中国科学院大连化学物理研究所_会议论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Qin HQ,Wu RA,Pan CS,et al. Profiling of N-linked Glycans from Human Serum with Oxidative Ordered Mesoporous Carbon[C]. 见:The 24th International Symposium on Micro-scale Bioseparation,. 中国. 2009-10-19.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [秦洪强]'s Articles
 [吴仁安]'s Articles
 [潘陈松]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [秦洪强]‘s Articles
 [吴仁安]‘s Articles
 [潘陈松]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace