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学科主题: 物理化学
题名: Studies on Bioactive Constituents of Traditional Chinese Medicine Introduced by Biological Fingerprinting Analysis
作者: Zhang JY(张建业) ;  Zhang LL(张灵利) ;  Kong L(孔亮) ;  YuKe ;  Wang Y(王芸) ;  YanXuebin ;  Lei XY(雷小元) ;  Hu LH(胡良海) ;  Zhu WG(朱为国) ;  LiuHongmin ;  Zou HF(邹汉法)
会议名称: The 24th International Symposium on Micro-scale Bioseparation,
会议日期: 2009-10-19
出版日期: 2009-10-19
会议地点: 中国
通讯作者: 邹汉法
部门归属: 十八室
主办者: 大连化物所
摘要: Screening of bioactive compounds in traditional Chinese medicines (TCMs) is an extremely challenge, and it’s also the basic and crucial problem to overcome for the modernization of TCMs. A novel method has been presented to resolve this problem potentially with the proposing of biological fingerprinting analysis. Biological fingerprinting analysis of TCMs is defined as the fingerprintings and their differences of the extracts of TCMs before and after interaction with the biological system [1]. Furthermore, biochromatography and two-dimensional-liquid chromatography (2D-LC) related with biochromatography can be regarded as a type of biological fingerprinting of TCMs [2]. The information which responding the interaction of the components from TCMs with the biological system drawing directly from the biological fingerprinting could guide the screening of the bioactive compounds. In the paper, biological fingerprintings of the aqueous extract of Flos Carthami with human serum albumin (HSA) and with various blood plasmas have been studied by the methods of microdialysis coupled with HPLC or centrifugal ultrafiltration coupled with HPLC. In addition, biochromatography with silica-bonded HSA as stationary phase and 2D-LC using an immobilized HSA column and a RP-HPLC column have also been investigated. LC-MS has been used to determine the structures of the components of the extract as well. According to the results of the biological fingerprinting analysis, some compounds with high contents in the herb and moderate binding rate with HSA or plasma have been selected as our target for further study. Isolation and purification of them were conducted and the structures of them have been determined. Binding constants and binding sites of them with HSA were also studied.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113856
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Zhang JY,Zhang LL,Kong L,et al. Studies on Bioactive Constituents of Traditional Chinese Medicine Introduced by Biological Fingerprinting Analysis[C]. 见:The 24th International Symposium on Micro-scale Bioseparation,. 中国. 2009-10-19.
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