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学科主题: 物理化学
题名: New Approach to Phosphopeptides Enrichment Using Titanium-Metal Oxide Composites and Metal Oxide Modified Mesoporous Silica
作者: Li XL(李秀玲) ;  Yan JY(闫竟宇) ;  Wan HH(万慧慧) ;  Ke YX(柯燕雄) ;  Liang XM(梁鑫淼)
会议名称: 24th International Symposium on Microscale Bioseparations
会议日期: 2009-10-18
出版日期: 2009-10-18
会议地点: 中国
其他题名: TiO2和氧化物修饰的介孔材料用于富集磷酸化多肽
通讯作者: 梁鑫淼
部门归属: 十八室
主办者: 中国科学院大连化物所
摘要: Reversible protein phosphorylation regulate most cellular processes such as signal transduction, metabolic maintenance and cell cycle. Analysis of this modifications presents formidable challenges due to their low abundance and the signal suppression of non-phosphorylated peptides. Thus, it is vital to enrich phosphopeptides from the highly complicated mixture prior to mass spectrometry (MS) analysis. In this study, different titanium-metal oxide composites with monodisperse uniform microsphere (~ 3µm) were synthesized by sol-gel method. Among these, seven titanium-metal oxide composites including TiO2-ZrO2 and TiO2-Al2O3 provided efficient enrichment of phosphopeptides and show higher specificity for phosphopeptides when compared with the commercial TiO2. In addition, we synthesized metal oxide (MxOy) coated mesoporous cellular foam structure (MCF) according to reported procedure. These materials were characterized with X-ray diffraction patterns, scanning electron microscopy, N2 sorption and UV-visible diffuse reflectance spectra and found their high surface area, large pore size and highly dispersed matel ions on the mesoporous silica surface. Isolation of phosphopeptides by MxOy on the mesoporous silica surface was demonstrated to be very specific and selective using model phosphoproteins as the test samples. Compared to the commonly used enrichment methods, this phosphopeptides enrichment strategy is more efficient and effective for both single- and multi-phosphopeptides.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113868
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Li XL,Yan JY,Wan HH,et al. New Approach to Phosphopeptides Enrichment Using Titanium-Metal Oxide Composites and Metal Oxide Modified Mesoporous Silica[C]. 见:24th International Symposium on Microscale Bioseparations. 中国. 2009-10-18.
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