Subject Area物理化学
New Approach to Phosphopeptides Enrichment Using Titanium-Metal Oxide Composites and Metal Oxide Modified Mesoporous Silica
Li XL(李秀玲); Yan JY(闫竟宇); Wan HH(万慧慧); Ke YX(柯燕雄); Liang XM(梁鑫淼)
Conference Name24th International Symposium on Microscale Bioseparations
Conference Date2009-10-18
Conference Place中国
Alternative TitleTiO2和氧化物修饰的介孔材料用于富集磷酸化多肽
Funding Organization中国科学院大连化物所
AbstractReversible protein phosphorylation regulate most cellular processes such as signal transduction, metabolic maintenance and cell cycle. Analysis of this modifications presents formidable challenges due to their low abundance and the signal suppression of non-phosphorylated peptides. Thus, it is vital to enrich phosphopeptides from the highly complicated mixture prior to mass spectrometry (MS) analysis. In this study, different titanium-metal oxide composites with monodisperse uniform microsphere (~ 3µm) were synthesized by sol-gel method. Among these, seven titanium-metal oxide composites including TiO2-ZrO2 and TiO2-Al2O3 provided efficient enrichment of phosphopeptides and show higher specificity for phosphopeptides when compared with the commercial TiO2. In addition, we synthesized metal oxide (MxOy) coated mesoporous cellular foam structure (MCF) according to reported procedure. These materials were characterized with X-ray diffraction patterns, scanning electron microscopy, N2 sorption and UV-visible diffuse reflectance spectra and found their high surface area, large pore size and highly dispersed matel ions on the mesoporous silica surface. Isolation of phosphopeptides by MxOy on the mesoporous silica surface was demonstrated to be very specific and selective using model phosphoproteins as the test samples. Compared to the commonly used enrichment methods, this phosphopeptides enrichment strategy is more efficient and effective for both single- and multi-phosphopeptides.
Document Type会议论文
Corresponding AuthorLiang XM(梁鑫淼)
Recommended Citation
GB/T 7714
Li XL,Yan JY,Wan HH,et al. New Approach to Phosphopeptides Enrichment Using Titanium-Metal Oxide Composites and Metal Oxide Modified Mesoporous Silica[C],2009:185/1.
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