Subject Area物理化学
Lysophosphatidylcholines profiling of plasma: isomers discrimination and biomarkers discovery in lung cancer
Dong J(董军); Zhang XL(张秀莉); Cai XM(蔡晓明); Zou LJ(邹丽娟); Xue XY(薛兴亚); Liang XM(梁鑫淼)
Conference Name24th International Symposium on Microscale Bioseparations
Conference Date2009-10-18
Conference Place中国
Alternative Title血浆中溶血卵磷脂的轮廓分析:肺癌中生物标记物的发现及异构体的鉴定
Funding Organization中国科学院大连化物所
AbstractLysophosphatidylcholines (lysoPCs) are a class of special structure endogenous compounds, which have the same polar head with different fatty acyls in chain lengths, fatty acyl position, degrees of saturation and different double bond location(s). The amount of lysoPCs isomers between healthy people and patients can be a clinical diagnostic indicator which may help to reveal pathophysiological changes. In this work, a method was developed to discriminate the lysoPCs by using reversed phase ultra-performance liquid chromatography coupled to quadrupole-time of flight mass spectrometry (UPLC/Q-TOF) with the utility of MSE function. Isomeric lysoPCs were distinguished based on the retention time and the peak intensity ratio of product ions. 28 plasma lysoPCs species were identified from human plasma. The plasma samples of 12 patients with lung cancer and 12 healthy persons were collected and analyzed. With principal component analysis (PCA), the identified LysoPCs metabolic profiles of lung cancer were investigated. Both ESI+ and ESI- results showed that lung cancer patients had the same 5 lysoPCs metabolic abnormality, they are sn-1 lyso16:0, sn-2 lysoPC 16:0, sn-1 lysoPC 18:0, sn-1 lysoPC 18:1 and sn-2 lysoPC 18:2. It is noteworthy that the function of isomers with the different fatty acyls position or different double bond location(s) related to lung cancer is different. This may help elucidate the action mechanism of lung cancer.
Document Type会议论文
Corresponding AuthorZhang XL(张秀莉); Liang XM(梁鑫淼)
Recommended Citation
GB/T 7714
Dong J,Zhang XL,Cai XM,et al. Lysophosphatidylcholines profiling of plasma: isomers discrimination and biomarkers discovery in lung cancer[C],2009:273/1.
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