Subject Area物理化学
CD bonded stationary phase in phosphopeptide fractionation and enrichment
Zhao YY(赵艳艳); Li XL(李秀玲); Yan JY(闫竟宇); Liang XM(梁鑫淼); Zhang YK(张玉奎)
Conference Name24th International Symposium on Microscale Bioseparations
Conference Date2009-10-18
Conference Place中国
Alternative Title环糊精键合固定相在磷酸化肽分段和富集的应用
Funding Organization中国科学院大连化物所
Abstracteversible protein phosphorylation regulates many significant cellular processes. Identification of the phosphorylation sites is vital to elucidate their biofunctions. To aid in phosphoproteome characterization, selective enrichent method, such as IMAC and TiO2, have been developed. However,high pH elution step is always used, which may degrade phosphopeptides. Furthermore, multiphosphopeptides bind strongly on TiO2 and are difficult to elute. More selective enrichment methods should be developed for phosphopeptide separation. Click CD stationary phase was synthesized and used to fractionate and enrich phosphopeptides in HILIC mode. Both fractionation and solid phase extraction (SPE) were carried out for separating phopshopeptides from tryptic α-casein. In fractionation, phosphopeptides are separated according to polarity, which is different from that with SCX or SAX. Phosphopeptides with same charge could be well resolved with the method. In SPE, CD stationary phase has similar selectivity to that with commercial TiO2. Furthermore, multiphosphopeptides could be well enriched and easily eluted from CD stationary phase, which are often unable to detect with TiO2. It is worthy of note that the whole enrichment process was carried out under low PH, which could avoid degradation of phosphopeptides. We demonstrate that the CD stationary phase be complementary to conventional method in phosphopeptide fractionation and enrichment.
Document Type会议论文
Corresponding AuthorLi XL(李秀玲); Liang XM(梁鑫淼)
Recommended Citation
GB/T 7714
Zhao YY,Li XL,Yan JY,et al. CD bonded stationary phase in phosphopeptide fractionation and enrichment[C],2009:277/1.
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