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学科主题: 物理化学
题名: Identification and Characterization of the low-abundant constituents of Radix isatidis by off-line 2D-LC coupled with Q-TOF MS
作者: Ceng J(曾静) ;  Guo ZM(郭志谋) ;  Zhang XL(张秀莉) ;  Xiao YS(肖远胜) ;  Wang CR(王超然) ;  Xue XY(薛兴亚) ;  Liang XM(梁鑫淼)
会议名称: 24th International Symposium on Microscale Bioseparations
会议日期: 2009-10-18
出版日期: 2009-10-18
会议地点: 中国
其他题名: 离线二维液相色谱-质谱分离鉴定板蓝根中的微量化合物
通讯作者: 梁鑫淼
部门归属: 十八室
主办者: 中国科学院大连化物所
摘要: Radix Isatidis (BanLanGen in Chinese) is the one of the most commonly used Traditional Chinese Medicine (TCMs) for its antiviral activity. But the constituents is unclear, especially the low-abundant ones. In this study, an orthogonal off-line two-dimensional high performance liquid chromatography (2D-LC) based on two different reversed-phase columns, a C18 column (XTerra MS C18) and a home-made polar-introduced C18 column (C18HC), provided with different molecular interaction was developed for the separation of Radix Isatidis. 1 The eluent was monitored by diode-array detection (DAD) and electrospray quadrupole time-of-flight tandem mass spectrometry (ESI-Q/TOF). Pure standards, accurate mass measurements and characteristic fragment ions were used to identify or tentatively characterize the structure of the separated compounds, including alkaloid, phenolic acid and phenyl propanoid. More than thirty compounds were identified or tentatively characterized based on their retention time, UV spectrometry and MS data. In this 2D-LC separation system, some low-abundant compounds covered by the major compounds were efficiently separated and identified for its highly separation resolution and sensitivity. For example, m/z 143 was identified as the molecular ion obviously in the 2D-LC analysis compared to the 1D-LC. As a result, the off-line 2D-LC-MS method in the present work was expected to be an effective technique to separate and identify the minor compounds for the advantage of reducing the influence of the signal suppression and providing more reliable structural information.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113896
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Ceng J,Guo ZM,Zhang XL,et al. Identification and Characterization of the low-abundant constituents of Radix isatidis by off-line 2D-LC coupled with Q-TOF MS[C]. 见:24th International Symposium on Microscale Bioseparations. 中国. 2009-10-18.
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