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学科主题: 物理化学
题名: Sample Preparation in Phosphoproteome Analysis
作者: Zou HF(邹汉法) ;  Ye ML(叶明亮)
会议名称: The 13rd Asia Chemical Congress
会议日期: 2009-9-14
出版日期: 2009-09-14
会议地点: 中国
通讯作者: 邹汉法
部门归属: 十八室
主办者: The Federation of Asian Chemical Societies
摘要: The elucidation of protein post-translational modifications, such as phosphorylation, remains a challenging analytical task of proteomic studies. Since many of the proteins targeted for phosphorylation are low in abundance and phosphorylation is typically sub-stochiometric, a prerequisite for their identification is the specific enrichment of phosphopeptide prior to mass spectrometric analysis. A new type of the immobilized metal ion affinity chromatography (IMAC) through the chelating interaction between phosphate groups on the polymer and Zr4+ and Ti4+ (Zr4+-and Ti4+-IMAC) has been developed for enriching phosphopeptides1,2. We also compared Zr4+- and Ti4+-IMAC to other enrichment methods including Fe3+-IMAC, TiO2 and ZrO2, and demonstrate superior selectivity and efficiency of Zr4+- and Ti4+-IMAC for the isolation and enrichment of phosphopeptides. Highly ordered mesoporous silica particles were modified with titanium phosphonate to selectively capture the phosphopeptides from complex peptide and protein mixtures3,4. The modified mesoporous silica particles were further used to enrich phosphopeptides from serum of hepatocellular carcinoma patients and healthy individuals and then analyzed with MALDI-TOFMS. The profiling of the serum phosphopeptides between the cancer patients and healthy persons was distinguishingly different, which indicated the potential ability of this technique for cancer diagnosis and biomarker discovery.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/113994
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Zou HF,Ye ML. Sample Preparation in Phosphoproteome Analysis[C]. 见:The 13rd Asia Chemical Congress. 中国. 2009-9-14.
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