DICP OpenIR
学科主题物理化学
Sample Preparation in Phosphoproteome Analysis
Zou HF(邹汉法); Ye ML(叶明亮)
会议名称The 13rd Asia Chemical Congress
会议日期2009-9-14
2009-09-14
会议地点中国
页码26/1
部门归属十八室
主办者The Federation of Asian Chemical Societies
英文摘要The elucidation of protein post-translational modifications, such as phosphorylation, remains a challenging analytical task of proteomic studies. Since many of the proteins targeted for phosphorylation are low in abundance and phosphorylation is typically sub-stochiometric, a prerequisite for their identification is the specific enrichment of phosphopeptide prior to mass spectrometric analysis. A new type of the immobilized metal ion affinity chromatography (IMAC) through the chelating interaction between phosphate groups on the polymer and Zr4+ and Ti4+ (Zr4+-and Ti4+-IMAC) has been developed for enriching phosphopeptides1,2. We also compared Zr4+- and Ti4+-IMAC to other enrichment methods including Fe3+-IMAC, TiO2 and ZrO2, and demonstrate superior selectivity and efficiency of Zr4+- and Ti4+-IMAC for the isolation and enrichment of phosphopeptides. Highly ordered mesoporous silica particles were modified with titanium phosphonate to selectively capture the phosphopeptides from complex peptide and protein mixtures3,4. The modified mesoporous silica particles were further used to enrich phosphopeptides from serum of hepatocellular carcinoma patients and healthy individuals and then analyzed with MALDI-TOFMS. The profiling of the serum phosphopeptides between the cancer patients and healthy persons was distinguishingly different, which indicated the potential ability of this technique for cancer diagnosis and biomarker discovery.
语种中文
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/113994
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法)
推荐引用方式
GB/T 7714
Zou HF,Ye ML. Sample Preparation in Phosphoproteome Analysis[C],2009:26/1.
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