DICP OpenIR
学科主题物理化学
Serum Biomarker Discovery by Quantification of Glycoproteins with Dimethyl Labeling of Non-glycopeptides
Chen R(陈锐); Ye ML(叶明亮); Zou HF(邹汉法)
会议名称25th International Symposium on Microscale Bioseparations MSB 2010
会议日期2010-3-21
2010-03-21
会议地点捷克
页码P011/2
部门归属十八室
主办者捷克分析化学研究所
英文摘要A robust, reproducible and high-throughput method was developed for the quantitative analysis of concentration changes of glycoproteins in human serum. This method consists of the capture of glycoproteins in serum samples and the release of non-glycopeptides by trypsin digestion of captured glycoproteins followed by dimethyl labeling the released non-glycopeptides. The labeled samples from different state are mixed and simultaneously analyzed by two dimensional LC-MS and the mass difference of the dimethyl labels is used to compare the peptide abundance in the different samples. This method showed almost the same specificity and sensitivity in glycoprotein identification compared with capture at glycopeptide level. By combination with isotope labeling of the released non-glycopeptides, reproducible quantification of glycoproteins was carried out to study the serum biomarker discovery of liver cancer. The differential abundance of proteins present at ng/ml levels was quantified with high confidence. The established method was applied to the analysis of human serum samples from healthy people and patients with primary hepatocellular carcinoma and a number of glycoproteins,including the currently used biomarker, alpha-fetoprotein were found with substantial concentration changes between normal and disease serum samples,which suggested this method was a good way to find potential biomarkers and demonstrated the potential application in the biomarker discovery for other diseases.
语种中文
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/114034
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法)
推荐引用方式
GB/T 7714
Chen R,Ye ML,Zou HF. Serum Biomarker Discovery by Quantification of Glycoproteins with Dimethyl Labeling of Non-glycopeptides[C],2010:P011/2.
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