中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 会议论文
学科主题: 物理化学
题名: Serum Biomarker Discovery by Quantification of Glycoproteins with Dimethyl Labeling of Non-glycopeptides
作者: Chen R(陈锐) ;  Ye ML(叶明亮) ;  Zou HF(邹汉法)
会议名称: 25th International Symposium on Microscale Bioseparations MSB 2010
会议日期: 2010-3-21
出版日期: 2010-03-21
会议地点: 捷克
通讯作者: 邹汉法
部门归属: 十八室
主办者: 捷克分析化学研究所
摘要: A robust, reproducible and high-throughput method was developed for the quantitative analysis of concentration changes of glycoproteins in human serum. This method consists of the capture of glycoproteins in serum samples and the release of non-glycopeptides by trypsin digestion of captured glycoproteins followed by dimethyl labeling the released non-glycopeptides. The labeled samples from different state are mixed and simultaneously analyzed by two dimensional LC-MS and the mass difference of the dimethyl labels is used to compare the peptide abundance in the different samples. This method showed almost the same specificity and sensitivity in glycoprotein identification compared with capture at glycopeptide level. By combination with isotope labeling of the released non-glycopeptides, reproducible quantification of glycoproteins was carried out to study the serum biomarker discovery of liver cancer. The differential abundance of proteins present at ng/ml levels was quantified with high confidence. The established method was applied to the analysis of human serum samples from healthy people and patients with primary hepatocellular carcinoma and a number of glycoproteins,including the currently used biomarker, alpha-fetoprotein were found with substantial concentration changes between normal and disease serum samples,which suggested this method was a good way to find potential biomarkers and demonstrated the potential application in the biomarker discovery for other diseases.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/114034
Appears in Collections:中国科学院大连化学物理研究所_会议论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Chen R,Ye ML,Zou HF. Serum Biomarker Discovery by Quantification of Glycoproteins with Dimethyl Labeling of Non-glycopeptides[C]. 见:25th International Symposium on Microscale Bioseparations MSB 2010. 捷克. 2010-3-21.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [陈锐]'s Articles
 [叶明亮]'s Articles
 [邹汉法]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [陈锐]‘s Articles
 [叶明亮]‘s Articles
 [邹汉法]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace