中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 会议论文
学科主题: 物理化学
题名: Cultivation of two species of Marine Microalgae for Targets Accumulation
作者: ChanghongYao、DinaFeng、ZhaoanChen、SongXue
会议名称: 2010 Internationnal Symposium on Advanced Biological EngineeringI
会议日期: 2010-7-23
出版日期: 2010-07-23
会议地点: 中国
其他题名: 两种海洋微藻培养积累目标产物
通讯作者: 薛松
部门归属: 十八室
主办者: 清华大学和日本东京工业大学
摘要: Starch constitutes a major carbon sink for CO2 fixation during the process of photosynthesis of microalgae. Some microalgae, primary green algae, have been proved to accumulate large amount of intracellular starch under stress conditions. It is strikingly valuable and important for production of algal-derived starch in large scale to meet the demand for renewable and clean energy (bioethanol, bio-hydrogen, etc.). A marine green microalga Tetraselmis subcordiformis was investigated its growth as well as intracellular starch accumulation in a 10L flat airlift photobioreactor (420×80×500 mm) with inside channel under continuous illumination (120~250 μ mol E • m-2 • s-1) at 30℃. 3% CO2- rich air was compressed into the photobioreactor to reach the aeration of 0.125 VVM. The results showed that under nitrogen limiting condition (1.18 mmol/L initial NO3-), T. subcordiformis was able to accumulate starch from initially 20% (dry weight based) to more than 50% within five days. The decline of chlorophyll florescence dynamics parameters might be attributed to the starch accumulation under the photosynthetic stressed condition. Microalgae lipids, as a source of renewable biofuels, have attracted more and more attentions due to their ability to produce biodiesel and hydrocarbon gas [5]. In order to improve the lipid content, the most effective culture condition was the depletion of the major nutrient nitrogen [6]. In our laboratory, we studied the supplement time of sodium nitrate and the effects of different nitrogen sources on cell growth and lipid accumulation of Isochrysis. zhangjiangensis. Using 0.6 L tubular photobioreactors (diameter5cm×height 70cm), 882µmol.l-1sodium nitrate was supplied in f/2 culture medium for 24h once, 48h once, 72h once, respectively. The initial cell density was 8×106cell.mL-1. The experiments were performed at 25±1ºC, 2%CO2 supply of 0.8 vvm and light/dark 14:10 at light intensity of 200µEm−2s−1. The highest biomass of 2.4± 0.6g.l-1was obtained when the medium was added every day. The highest total lipid content 44.9 ± 0.1% and lipid productivity 119.7 ± 29.9 mg.l-1day-1 were achieved when nitrogen source is supplied every day. The variation in fatty acid composition of three cultures is significant and sixteen kinds of fatty acids are monitored in the cultures for. The highest biomass productivity was obtained when 882µmol.l-1urea was supplied once a day, with a biomass concentration of 3.09±0.27 g/l and a lipid content of 36.1±1.5%.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/114108
Appears in Collections:中国科学院大连化学物理研究所_会议论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
ChanghongYao、DinaFeng、ZhaoanChen、SongXue. Cultivation of two species of Marine Microalgae for Targets Accumulation[C]. 见:2010 Internationnal Symposium on Advanced Biological EngineeringI. 中国. 2010-7-23.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [ChanghongYao、DinaFeng、ZhaoanChen、SongXue]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [ChanghongYao、DinaFeng、ZhaoanChen、SongXue]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace