DICP OpenIR
学科主题物理化学
Ionic Liquids Based Solvent System for Rat brain membrane proteome analysis
Sun LL(孙良亮); Tao DY(陶定银); Zhao Q(赵群); Zhang YK(张玉奎)
会议名称全球第九届人类蛋白质组学会议
会议日期2010-9-19
2010-09-19
会议地点澳大利亚
页码121/2
部门归属生物技术部
主办者University of New South Wales
英文摘要The solubility and digestion efficiency are two crucial factors that might affect the identification of integral membrane proteins (IMPs). In our recent work, a novel solvent system, 1% (v/v) ionic liquids (ILs), 1-butyl-3-methyl imidazolium tetrafluoroborate (BMIM BF4), dissolved in NH4HCO3 buffer (pH 8.3), was applied for rat brain proteome analysis. Compared to commonly used SDS and methanol methods, the identified IMPs number from rat brain, analyzed by μRPLC-ESIMS/ MS, was improved by more than 3 times, which might be due to the good solubilizing ability and biocompatibility of BMIM BF4 for tryptic digestion. Furthermore, compared to Rapigest and urea methods, with BMIM BF4 method, the numberof identified IMPs could be improved 25% and 80%, respectively, which should be contributed to the good solubilizing ability and high thermal stability of such ILs. All these results demonstrate that BMIM BF4 method is of advantages for membrane proteome analysis. Such a method was applied for the further analysis of insoluble proteins extracted from rat brain. By 2D-nano-SCX-RPLC-ESI-MS/MS, in triplicate runs, 2565 nonredundant proteins were identified, and 716 proteins were of at least one predicted transmembrane domain. In addition, through the membrane proteome profiling of human liver microsome, 1830 nonredundant proteins were identified, including 31 CYP enzymes and 16 UGT enzymes. Our experimental results demonstrate that the novel solvent system containing BMIM BF4 is promising for the large-scale identification of IMPs.
语种中文
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/114162
专题中国科学院大连化学物理研究所
通讯作者Zhang YK(张玉奎)
推荐引用方式
GB/T 7714
Sun LL,Tao DY,Zhao Q,et al. Ionic Liquids Based Solvent System for Rat brain membrane proteome analysis[C],2010:121/2.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[孙良亮]的文章
[陶定银]的文章
[赵群]的文章
百度学术
百度学术中相似的文章
[孙良亮]的文章
[陶定银]的文章
[赵群]的文章
必应学术
必应学术中相似的文章
[孙良亮]的文章
[陶定银]的文章
[赵群]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。