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学科主题: 物理化学
题名: CLASSIFIED CELL CULTURE FOR METABOLISM STUDY OF TWO BIOACTIVE ALKALOIDS IN THE MARINE SPONGE
作者: Song YF(宋悦凡) ;  Qu Y(曲翊) ;  Cao XP(曹旭鹏) ;  Xue S(薛松) ;  Zhang W(张卫)
会议名称: 9th International Marine Biotechnology Conference
会议日期: 2010-10-8
出版日期: 2010-10-08
会议地点: 中国
其他题名: 小轴海绵两种活性生物碱基于细胞分类培养的代谢研究
通讯作者: 薛松
部门归属: 十八室
主办者: 青岛海洋所
摘要: Sponges are the richest resources of marine natural products. During the past decades, much attention has been paid on finding novel sponge-derived compounds and examining their potential pharmacological activities [1]. Although many sponge-derived compounds are excellent drug candidates, wild harvest of sponges is insufficient to supply the bioactive compounds in quantity for clinical researches and commercial demands. Sponge-derived bioactives production by in vitro sponge cell culture might be feasible to solve this supply problem. Culture of sponge primmorphs [2], a special sponge cell aggregates, is a promising system to study sponge cell culture as well as sponge cell biology. In this research, we developed a sponge cell fraction and purification technique, by which three types of primmorph cultures (named spherulous cell primmorphs, 29g primmorphs, and 110g primmorphs) were prepared from different dominant cell types. During the 30-day culture, the three types of primmorph culture showed different proliferation and differentiation dynamics in terms of morphological characteristics, attachment features and metabolic behaviors of two bioactive alkaloids (Debromohymenialdisine (DBH) and Hymenialdisine (HD), located in spherulous cells (submitted)). The concentration of DBH and HD in spherulous cell-dominant primmorphs declined during the culture. The 110g primmorphs were more likely to attach and spread on the bottom of Petri dishes. The 29g primmorphs showed an increasing trend in DBH and HD concentration, which make it a good model for further metabolic manipulation research.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/114198
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Song YF,Qu Y,Cao XP,et al. CLASSIFIED CELL CULTURE FOR METABOLISM STUDY OF TWO BIOACTIVE ALKALOIDS IN THE MARINE SPONGE[C]. 见:9th International Marine Biotechnology Conference. 中国. 2010-10-8.
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