DICP OpenIR
Subject Area物理化学
CLASSIFIED CELL CULTURE FOR METABOLISM STUDY OF TWO BIOACTIVE ALKALOIDS IN THE MARINE SPONGE
Song YF(宋悦凡); Qu Y(曲翊); Cao XP(曹旭鹏); Xue S(薛松); Zhang W(张卫)
Conference Name9th International Marine Biotechnology Conference
Conference Date2010-10-8
2010-10-08
Conference Place中国
Alternative Title小轴海绵两种活性生物碱基于细胞分类培养的代谢研究
Pages77/2
Department十八室
Funding Organization青岛海洋所
AbstractSponges are the richest resources of marine natural products. During the past decades, much attention has been paid on finding novel sponge-derived compounds and examining their potential pharmacological activities [1]. Although many sponge-derived compounds are excellent drug candidates, wild harvest of sponges is insufficient to supply the bioactive compounds in quantity for clinical researches and commercial demands. Sponge-derived bioactives production by in vitro sponge cell culture might be feasible to solve this supply problem. Culture of sponge primmorphs [2], a special sponge cell aggregates, is a promising system to study sponge cell culture as well as sponge cell biology. In this research, we developed a sponge cell fraction and purification technique, by which three types of primmorph cultures (named spherulous cell primmorphs, 29g primmorphs, and 110g primmorphs) were prepared from different dominant cell types. During the 30-day culture, the three types of primmorph culture showed different proliferation and differentiation dynamics in terms of morphological characteristics, attachment features and metabolic behaviors of two bioactive alkaloids (Debromohymenialdisine (DBH) and Hymenialdisine (HD), located in spherulous cells (submitted)). The concentration of DBH and HD in spherulous cell-dominant primmorphs declined during the culture. The 110g primmorphs were more likely to attach and spread on the bottom of Petri dishes. The 29g primmorphs showed an increasing trend in DBH and HD concentration, which make it a good model for further metabolic manipulation research.
Language中文
Document Type会议论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/114198
Collection中国科学院大连化学物理研究所
Corresponding AuthorXue S(薛松)
Recommended Citation
GB/T 7714
Song YF,Qu Y,Cao XP,et al. CLASSIFIED CELL CULTURE FOR METABOLISM STUDY OF TWO BIOACTIVE ALKALOIDS IN THE MARINE SPONGE[C],2010:77/2.
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