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学科主题: 物理化学
题名: Description and phylogenetic study of Hymeniacidon perlevis in North China
作者: Cao H(曹恒) ;  Cao XP(曹旭鹏) ;  Xue S(薛松) ;  Zhang W(张卫)
会议名称: 9th International Marine Biotechnology Conference
会议日期: 2010-10-8
出版日期: 2010-10-08
会议地点: 中国
其他题名: 基于分子系统发育水平的中国北方大连海域繁茂膜海绵的研究
通讯作者: 薛松
部门归属: 十八室
主办者: 中国科学院、国家自然科学基金委员会以及青岛市人民政府支持下,由中国科学院海洋研究所主办
摘要: As one of the most common species in the tidal and shallow subtidal in Europe, Hymeniacidon perlevis was first described by Montagu in 1818[1]. H. perlevis in North China has been a model marine sponge for natural products, explants cultivation, cell cultivation, biosilicification of silicatein and sponge-associated actinobacteria since 2002. The diversity of morphology is rich and it brings lots of trouble to get a confident comparative results. The traditional identification method is mostly based on morphology and spiclues (skeloton) of sponge. We hope to introduce the modern biotechniques to identify the sponges according to their molecular markers as well as their morphology. It’s reported that the H. perlevis is always confused with H. sinapium, which is also popular in East Asia[2]. Here we compared three common H. perlevis with different color or locality in Dalian. Two common genetic markers (18S rDNA and COI) were used to investigate the difference between these sponges in this paper. The specimen of H. sinapium from Japan is used to compare with H. perlevis. The results show H. perlevis with different morphology characters in Dalian are the same genus; and the 658 base pairs(bp) COI fragments of H. perlevis in Dalian and H. sinapium in Japan are one bp difference, but 18S rDNA fragments of these two species show a little difference (0.28% in 1761bp). From the examples of H. perlevis and H. sinapium we demonstrate that barcoding facilitates rapid assessment of biodiversity, radiation patterns and the detection of cryptic speciation. We also prove that due to the low evolutionary rates in these two species, the COI standard barcoding fragment should be used in combination with another DNA marker in order to achieve unambiguous taxonomic identification at species level.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/114200
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Cao H,Cao XP,Xue S,et al. Description and phylogenetic study of Hymeniacidon perlevis in North China[C]. 见:9th International Marine Biotechnology Conference. 中国. 2010-10-8.
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