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学科主题: 物理化学
题名: IMPROVEMENT OF LIPID PRODUCTIVITY REGULATED BY NITROGEN SOURCE SUPPLEMENT ON A 24H BASIS IN MARINE GOLDEN ALGAE ISOCHRYSIS ZHANGJIANGENSIS
作者: Feng DN(冯迪娜) ;  Chen ZA(陈兆安) ;  Zhang W(张卫) ;  Xue S(薛松)
会议名称: 9th International Marine Biotechnology Conference
会议日期: 2010-10-8
出版日期: 2010-10-08
会议地点: 中国
其他题名: 氮源的补加对于提高湛江等鞭金藻脂肪含量的研究
通讯作者: 薛松
部门归属: 十八室
主办者: 中国科学院、国家自然科学基金委员会以及青岛市人民政府支持下,由中国科学院海洋研究所主办
摘要: Microalgae lipids, as a source of renewable biofuels, have attracted more and more attentions due to their ability to produce biodiesel and hydrocarbon gas [1]. In order to improve the lipid content, the most effective culture condition was the depletion of the major nutrients nitrogen [2]. In our laboratory, we studied the supplement period of sodium nitrate and the effect of different nitrogen source on cell growth and lipid accumulation of Isochrysis. zhangjiangensis. Using 0.6 L tubula photobioreactors (diameter5cm×height 42cm), 882µmol.l-1sodium nitrate was supplied in f/2 culture medium for 24h once, 48h once, 72h once, respectively. The initial cell density was 8×106cell.mL-1. The experiments were performed at 25±1ºC, 2%CO2 supply of 0.8 vvm and light/dark 14:10 at light intensity of 200µEm−2s−1. The highest biomass of 2.4± 0.6g.l-1was obtained at the intermittent-supplying for 24h once. Surprisingly, with N-deprivation regulation, the difference is that the highest total lipid content 44.9 ± 0.1% and lipid productivity 119.7 ± 29.9 mg.l-1day-1 are received when nitrogen source is supplied every 24h. The variation in fatty acid composition of three cultures is significant and sixteen kinds of fatty acids are monitored in the cultures for intermittent-supplying of 24h. The highest biomass productivity was obtained when 882µmol.l-1urea was supplied for 24h once, with a biomass concentration of 3.09±0.27 g.l-1 and a lipid content of 36.1±1.5%.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/114202
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Feng DN,Chen ZA,Zhang W,et al. IMPROVEMENT OF LIPID PRODUCTIVITY REGULATED BY NITROGEN SOURCE SUPPLEMENT ON A 24H BASIS IN MARINE GOLDEN ALGAE ISOCHRYSIS ZHANGJIANGENSIS[C]. 见:9th International Marine Biotechnology Conference. 中国. 2010-10-8.
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