Subject Area物理化学
New Methods and Platforma for Liquid Phase Based Proteome Separation and Identification
Zhang LH(张丽华); Zhou Y(周愿); Yuan HM(袁辉明); Zhu GJ(朱贵杰); Qiao XQ(乔小强); Sun LL(孙良亮); Liang Z(梁振); Zhang YK(张玉奎)
Conference Name35th International Symposium on High Performance Liquid Phase Separations and Related Techniques
Conference Date2010-6-19
Conference PlaceUSA
Funding OrganizationCalifornia Separation Science Society
AbstractThe complexity of proteome brings great challenge and opportunity to promote the development of new techniques for liquid phase based proteome analysis. In our recent study, with newly developed methods and platforms, great improvement on proteome sample preparation, separation and identification was made. To decrease the dynamic range of proteome, human scFv display phage library, with 106 different types scFv fragment, and ca. 106 phages for each type, was immobilized on amino group modified magnetic microspheres, and applied into the treament of human the specific affinity, instead of the amount, Therefore, through sample overloading, simulataneously, high abundace proteins could be removal, and low abundace ones could be enriched, By such a method, the identidied protein number from plasma was doubled.
Document Type会议论文
Corresponding AuthorZhang LH(张丽华)
Recommended Citation
GB/T 7714
Zhang LH,Zhou Y,Yuan HM,et al. New Methods and Platforma for Liquid Phase Based Proteome Separation and Identification[C],2010:30/2.
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