中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 会议论文
学科主题: 物理化学
题名: A type of characteristic peroxisome in the spherulous cells of the marine sponge Axinella sp
作者: Song YF(宋悦凡) ;  Qu Y(曲翊) ;  Zhang JY(张锦友) ;  Cao XP(曹旭鹏) ;  Zhang W(张卫)
会议名称: 14th International Biotechnology Symposium and Exhibition
会议日期: 2010-9-14
出版日期: 2010-09-14
会议地点: 意大利
通讯作者: 曹旭鹏
部门归属: 十八室
主办者: 意大利
摘要: A type of characteristic peroxisome in the spherulous cells of the marine sponge Axinella sp Debromohymenialdisine (DBH) and hymenialdisine (HD) are two bioactive alkaloids with pharmacological activities for treating osteoarthritis and Alzheimer’s disease. These compounds had been isolated from the marine sponge Axinella sp., and were recently cellular localized particularly in the spherulous cells. Establishing a marker for this type of cells is required for studies on cell differentiation and metabolism regulation of the two alkaloids in in vitro cell culture system. In this research, a type of peroxisome and the relevant peroxidase was detected as markers exclusively in spherulous cells. First, the peroxidase activities in different cell fractions were examined using a cell separation protocol and followed by cytochemical staining with 3,3 -diaminobenzidine (DAB). With the separation protocol, the sponge cells were divided into three fractions, consisted of enriched spherulous cells, the large sponge cells, and the small sponge cells, respectively. The subsequent staining exhibited a distinguishing feature of dark brown granules in cytoplasm exclusively in spherulous cells. Then the transmission electronic microscopy (TEM) was employed to examine the inner structure of the spherulous cells. Two kinds of inclusions were observed inside, of which the larger ones, with a regular oval shape, smooth and even in texture, were found to be the responsible peroxisomes in DAB staining, as they showed consistency in dimension and amount with the positive stained granules observed in light microscopy. Finally, a native–PAGE with protein extracts from different cell fractions was conducted to show the relevant peroxidase. The assay displayed a solely distinctive band with peroxidase activity in the spherulous cell extracts, with a molecular weight of 220kd. This was the first application of DAB staining in sponge and worked out a characteristic peroxisome in spherulous cells. These results would benefit cell identification and also initiate the studies on peroxisome function and their role in DBH/HD metabolism.
语种: 中文
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/114500
Appears in Collections:中国科学院大连化学物理研究所_会议论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Song YF,Qu Y,Zhang JY,et al. A type of characteristic peroxisome in the spherulous cells of the marine sponge Axinella sp[C]. 见:14th International Biotechnology Symposium and Exhibition. 意大利. 2010-9-14.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [宋悦凡]'s Articles
 [曲翊]'s Articles
 [张锦友]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [宋悦凡]‘s Articles
 [曲翊]‘s Articles
 [张锦友]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace