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学科主题: 物理化学
题名: Engineering functional chitosan for delivery of drugs or RNAs
作者: Liu XD(刘袖洞) ;  Yang Y(杨艳) ;  Zhou HF(周火飞) ;  Yu WT(于炜婷) ;  Zheng JN(郑佳妮) ;  Zhang DM(张德蒙) ;  Ma XJ(马小军)
会议名称: 3rd International Symposium Cellular Delivery of Theraputic Macromolecules
会议日期: 2010-6-26
出版日期: 2010-06-26
会议地点: UK
通讯作者: 刘袖洞 ;  马小军
部门归属: 十八室
主办者: Cardiff University
摘要: In the last decade, considerable studies on preparation of nanocarriers with cationic liposomes or polymers have been reported for intracellular delivery of DNA and siRNA [1]. The particle uptake has been provn through several kinds of endocytosis pathways, but the uptake efficiency varies depending on the property of carrier materials, particle size, and cell types. Using biocompatible and biodegradable chitosan (CTS) as carrier material, we designed and synthesized functional chitosan derivatives (such as amphiphilic CTS, ligand-targeted CTS), and then developed different technology to prepare CTS nanoparticles for the potential application on loading, delivering and releasing anti-cancer drugs or RNA therapeutics (siRNA and microRNA). In one way, we firstly conjugated a fatty acid (LA) to CTS to get amphiphilc CTS-LA, and then synthezised CTS-LA-TM by quaternization. Subsequently nanoparticles with size less than 200 nm can be easily formed by self-assembly of CS-LA-TM in biological solution or neutral solution [2], which loaded PTX with encapsulation efficiency of 60-90% and showed sustained release in 1 week without burst release. In the other way, we formulated CTS-RNAs (siRNA or microRNA) nanoparticles by directly complexation. The nanoparticles with the size of 120-200 nm and surface charge of ~20 mV showed complex stability and efficiency of protecting RNAs from RNase degradation. The nanoparticles can transfer and protect entrapped RNAs into cells in 2-4 h without apparent critical cytotoxicity. Moreover, cell adhesive peptide GRGDY has been grafted to CTS by photosensitive crosslinker [3], and PEGylation has been carried out for the target transportation to tumor cells with over-expressed integrin receptors and for efficient delivery of drugs or RNA therapeutics.
语种: 中文
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内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/114514
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Liu XD,Yang Y,Zhou HF,et al. Engineering functional chitosan for delivery of drugs or RNAs[C]. 见:3rd International Symposium Cellular Delivery of Theraputic Macromolecules. UK. 2010-6-26.
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