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学科主题: 物理化学
题名: Purification and characterization of a hydrogenase from the marine green alga Tetraselmis subcordiformis
作者: Fan, Fei1, 2;  Chen, Zhaoan1;  Li, Wei3;  Cao, Xupeng1;  Xue, Song1;  Zhang, Wei1, 4
通讯作者: 张卫
关键词: Characterization ;  Hydrogenase ;  Marine green alga ;  Purification ;  Tetraselmis subcordiformis
刊名: PROCESS BIOCHEMISTRY
发表日期: 2011-05-01
DOI: 10.1016/j.procbio.2011.02.005
卷: 46, 期:5, 页:1212-1215
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1812
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Engineering, Chemical
摘要: Purification and characterization of a hydrogenase from the marine green alga Tetraselmis subcordiformis
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Engineering
英文摘要: Soluble hydrogenase from a marine green alga, Tetraselmis subcordiformis, was purified to homogeneity and characterized under strict anaerobic conditions. After a 4 h dark, anaerobic adaptation period for the algal culture, the hydrogenase showed a maximum in vitro hydrogen production activity of 148 nmol H(2)/(mu g Chl a x h). The enzyme was purified 362-fold by a three-step process, and using reduced methyl viologen as the electron donor its activity was 61.67 U/mg at 25 degrees C. The optimum temperature and pH value for hydrogen production were 55 degrees C and 7.5, respectively. The N-terminal amino acid sequence of this 46 kDa hydrogenase was determined, and it showed no homology to any known hydrogenase sequence. (C) 2011 Elsevier Ltd. All rights reserved.
关键词[WOS]: CHLAMYDOMONAS-REINHARDTII ;  PLATYMONAS-SUBCORDIFORMIS ;  SCENEDESMUS-OBLIQUUS ;  EVOLUTION
语种: 英语
WOS记录号: WOS:000290466800026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115193
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Dalian Fisheries Univ, Dept Food Engn, Dalian 116023, Peoples R China
4.Flinders Univ S Australia, Sch Med, Flinders Ctr Marine Bioproc & Bioprod, Adelaide, SA 5042, Australia
5.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Adelaide, SA 5042, Australia

Recommended Citation:
Fan, Fei,Chen, Zhaoan,Li, Wei,et al. Purification and characterization of a hydrogenase from the marine green alga Tetraselmis subcordiformis[J]. PROCESS BIOCHEMISTRY,2011,46(5):1212-1215.
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