DICP OpenIR
学科主题物理化学
Enrichment of peptides containing consensus sequence by an enzymatic approach for targeted analysis of proteins
Wang, Chunli1,2; Ye, Mingliang1; Han, Guanghui1,2; Chen, Rui1,2; Zhang, Manyu1,2; Jiang, Xinning1,2; Cheng, Kai1,2; Wang, Fangjun1,2; Zou, Hanfa1; Zou HF(邹汉法); Ye ML(叶明亮)
关键词Ms Protein Kinases Proteomics Methods Selective Isolation Targeted Analysis Technology
刊名PROTEOMICS
2011-09-01
ISSN1615-9853
DOI10.1002/pmic.201100041
11期:17页:3578-3581
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1,1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]MASS-SPECTROMETRY ; PROTEOME ANALYSIS ; TRYPTIC PEPTIDES ; IDENTIFICATION ; RESIDUES ; TOOL
英文摘要Enrichment of peptides containing consensus sequence by an enzymatic approach for targeted analysis of proteins; Multiple residues with consensus sequence, i.e. motif, on proteins are closely related to protein function. However, there is no effective method for targeted analysis of such proteins. The challenge for analysis of these classes of proteins by MS is how to selectively enrich peptides containing consensus sequence from protein digest. Although enrichment of peptides containing one type of amino acid residue was successfully achieved by chemically labeling followed by chromatographic isolation, however, it is almost impossible to label and isolate signature peptides containing multiple residues with consensus sequence by chemical approach. Herein, we developed an enzymatic approach based on the specific recognition between enzyme and its substrates to enrich such peptides. This approach was realized by modification of a residue in the consensus sequence via enzyme that can recognize the sequence followed by the isolation of the modified peptides. cAMP-dependent protein kinase was used to validate this approach and 168 peptides containing consensus motif were identified with selectivity of 67.2%. Those peptides resulted in the identification of 88 proteins with consensus sequence from serum sample. As this motif-oriented peptide enrichment approach allows targeted analysis of a subset of proteins with consensus sequence, it will have broad application in biological studies.
语种英语
WOS记录号WOS:000295217400014
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/115262
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Ye ML(叶明亮)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Wang, Chunli,Ye, Mingliang,Han, Guanghui,et al. Enrichment of peptides containing consensus sequence by an enzymatic approach for targeted analysis of proteins[J]. PROTEOMICS,2011,11(17):3578-3581.
APA Wang, Chunli.,Ye, Mingliang.,Han, Guanghui.,Chen, Rui.,Zhang, Manyu.,...&叶明亮.(2011).Enrichment of peptides containing consensus sequence by an enzymatic approach for targeted analysis of proteins.PROTEOMICS,11(17),3578-3581.
MLA Wang, Chunli,et al."Enrichment of peptides containing consensus sequence by an enzymatic approach for targeted analysis of proteins".PROTEOMICS 11.17(2011):3578-3581.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
2011w5xbylqdLz.pdf(150KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Chunli]的文章
[Ye, Mingliang]的文章
[Han, Guanghui]的文章
百度学术
百度学术中相似的文章
[Wang, Chunli]的文章
[Ye, Mingliang]的文章
[Han, Guanghui]的文章
必应学术
必应学术中相似的文章
[Wang, Chunli]的文章
[Ye, Mingliang]的文章
[Han, Guanghui]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。