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学科主题: 物理化学
题名: Enrichment of peptides containing consensus sequence by an enzymatic approach for targeted analysis of proteins
作者: Wang, Chunli1, 2;  Ye, Mingliang1;  Han, Guanghui1, 2;  Chen, Rui1, 2;  Zhang, Manyu1, 2;  Jiang, Xinning1, 2;  Cheng, Kai1, 2;  Wang, Fangjun1, 2;  Zou, Hanfa1
通讯作者: 邹汉法 ;  叶明亮
关键词: MS ;  Protein kinases ;  Proteomics methods ;  Selective isolation ;  Targeted analysis ;  Technology
刊名: PROTEOMICS
发表日期: 2011-09-01
DOI: 10.1002/pmic.201100041
卷: 11, 期:17, 页:3578-3581
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology
摘要: Enrichment of peptides containing consensus sequence by an enzymatic approach for targeted analysis of proteins
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Multiple residues with consensus sequence, i.e. motif, on proteins are closely related to protein function. However, there is no effective method for targeted analysis of such proteins. The challenge for analysis of these classes of proteins by MS is how to selectively enrich peptides containing consensus sequence from protein digest. Although enrichment of peptides containing one type of amino acid residue was successfully achieved by chemically labeling followed by chromatographic isolation, however, it is almost impossible to label and isolate signature peptides containing multiple residues with consensus sequence by chemical approach. Herein, we developed an enzymatic approach based on the specific recognition between enzyme and its substrates to enrich such peptides. This approach was realized by modification of a residue in the consensus sequence via enzyme that can recognize the sequence followed by the isolation of the modified peptides. cAMP-dependent protein kinase was used to validate this approach and 168 peptides containing consensus motif were identified with selectivity of 67.2%. Those peptides resulted in the identification of 88 proteins with consensus sequence from serum sample. As this motif-oriented peptide enrichment approach allows targeted analysis of a subset of proteins with consensus sequence, it will have broad application in biological studies.
关键词[WOS]: MASS-SPECTROMETRY ;  PROTEOME ANALYSIS ;  TRYPTIC PEPTIDES ;  IDENTIFICATION ;  RESIDUES ;  TOOL
语种: 英语
WOS记录号: WOS:000295217400014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115262
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Wang, Chunli,Ye, Mingliang,Han, Guanghui,et al. Enrichment of peptides containing consensus sequence by an enzymatic approach for targeted analysis of proteins[J]. PROTEOMICS,2011,11(17):3578-3581.
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