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学科主题: 物理化学
题名: Size-Selective Enrichment of N-Linked Glycans Using Highly Ordered Mesoporous Carbon Material and Detection by MALDI-TOF MS
作者: Qin, Hongqiang1, 2;  Zhao, Liang1, 2;  Li, Ruibin1, 2;  Wu, Ren'an1;  Zou, Hanfa1
通讯作者: 邹汉法
刊名: ANALYTICAL CHEMISTRY
发表日期: 2011-10-15
DOI: 10.1021/ac201198q
卷: 83, 期:20, 页:7721-7728
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1,1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
摘要: Size-Selective Enrichment of N-Linked Glycans Using Highly Ordered Mesoporous Carbon Material and Detection by MALDI-TOF MS
研究领域[WOS]: Chemistry
英文摘要: Many diseases are characterized by the changes of either glycan structure or glycosylation site of glycoproteins. The glycan profiling can provide the overview of glycosylation in despite of the absence of the glycosylation sites, which in turn simplifies the complexity of disease diagnosis. Herein, we describe a simple method to profile the N-linked glycans by MALDI-TOF MS with the enrichment using oxidized ordered mesoporous carbon, taking advantages of the size-exclusive effect of mesopore against proteins as well as the interaction between glycans and carbon. Twenty four N-linked glycans derived from ovalbumin could be efficiently detected with high signal-to-noise (SIN) ratios and sufficient peak intensities. In the analysis of complex serum samples, 32 N-linked glycans could be profiled, and 5 (4 core-fucosylated glycans) of them were distinguished from liver cancer and healthy samples.
关键词[WOS]: HEPATOCELLULAR-CARCINOMA ;  AFFINITY-CHROMATOGRAPHY ;  SILICA PARTICLES ;  LIVER-CANCER ;  GLYCOSYLATION ;  OLIGOSACCHARIDES ;  GLYCOPROTEINS ;  GLYCOPEPTIDES ;  PROTEOLYSIS ;  EXTRACTION
语种: 英语
WOS记录号: WOS:000295817500022
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115282
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Qin, Hongqiang,Zhao, Liang,Li, Ruibin,et al. Size-Selective Enrichment of N-Linked Glycans Using Highly Ordered Mesoporous Carbon Material and Detection by MALDI-TOF MS[J]. ANALYTICAL CHEMISTRY,2011,83(20):7721-7728.
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