DICP OpenIR
学科主题物理化学
Size-Selective Enrichment of N-Linked Glycans Using Highly Ordered Mesoporous Carbon Material and Detection by MALDI-TOF MS
Qin, Hongqiang1,2; Zhao, Liang1,2; Li, Ruibin1,2; Wu, Ren'an1; Zou, Hanfa1; Zou HF(邹汉法)
刊名ANALYTICAL CHEMISTRY
2011-10-15
ISSN0003-2700
DOI10.1021/ac201198q
83期:20页:7721-7728
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1,1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]HEPATOCELLULAR-CARCINOMA ; AFFINITY-CHROMATOGRAPHY ; SILICA PARTICLES ; LIVER-CANCER ; GLYCOSYLATION ; OLIGOSACCHARIDES ; GLYCOPROTEINS ; GLYCOPEPTIDES ; PROTEOLYSIS ; EXTRACTION
英文摘要Size-Selective Enrichment of N-Linked Glycans Using Highly Ordered Mesoporous Carbon Material and Detection by MALDI-TOF MS; Many diseases are characterized by the changes of either glycan structure or glycosylation site of glycoproteins. The glycan profiling can provide the overview of glycosylation in despite of the absence of the glycosylation sites, which in turn simplifies the complexity of disease diagnosis. Herein, we describe a simple method to profile the N-linked glycans by MALDI-TOF MS with the enrichment using oxidized ordered mesoporous carbon, taking advantages of the size-exclusive effect of mesopore against proteins as well as the interaction between glycans and carbon. Twenty four N-linked glycans derived from ovalbumin could be efficiently detected with high signal-to-noise (SIN) ratios and sufficient peak intensities. In the analysis of complex serum samples, 32 N-linked glycans could be profiled, and 5 (4 core-fucosylated glycans) of them were distinguished from liver cancer and healthy samples.
语种英语
WOS记录号WOS:000295817500022
引用统计
被引频次:48[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/115282
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Qin, Hongqiang,Zhao, Liang,Li, Ruibin,et al. Size-Selective Enrichment of N-Linked Glycans Using Highly Ordered Mesoporous Carbon Material and Detection by MALDI-TOF MS[J]. ANALYTICAL CHEMISTRY,2011,83(20):7721-7728.
APA Qin, Hongqiang,Zhao, Liang,Li, Ruibin,Wu, Ren'an,Zou, Hanfa,&邹汉法.(2011).Size-Selective Enrichment of N-Linked Glycans Using Highly Ordered Mesoporous Carbon Material and Detection by MALDI-TOF MS.ANALYTICAL CHEMISTRY,83(20),7721-7728.
MLA Qin, Hongqiang,et al."Size-Selective Enrichment of N-Linked Glycans Using Highly Ordered Mesoporous Carbon Material and Detection by MALDI-TOF MS".ANALYTICAL CHEMISTRY 83.20(2011):7721-7728.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
2011QqRJrEIeUT.pdf(1142KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Qin, Hongqiang]的文章
[Zhao, Liang]的文章
[Li, Ruibin]的文章
百度学术
百度学术中相似的文章
[Qin, Hongqiang]的文章
[Zhao, Liang]的文章
[Li, Ruibin]的文章
必应学术
必应学术中相似的文章
[Qin, Hongqiang]的文章
[Zhao, Liang]的文章
[Li, Ruibin]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。