DICP OpenIR
学科主题物理化学
Rapid screening of phenylketonuria using a CD microfluidic device
Chen, Bin1; Zhou, Xiaomian1; Li, Chunyu2; Wang, Qiuping1; Liu, Dayu1; Lin, Bingcheng2; XiaomianZhou; DayuLiu
关键词Phenylketonuria Dna Hybridization Cd Microfluidic Chip Reciprocating Flow
刊名JOURNAL OF CHROMATOGRAPHY A
2011-04-08
ISSN待补充
DOI10.1016/j.chroma.2011.02.001
1218期:14页:1907-1912
收录类别SCI
文章类型Article
部门归属18
项目归属1807
产权排名2,3
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PHENYLALANINE-HYDROXYLASE GENE ; POLYMERASE-CHAIN-REACTION ; DNA ; MUTATIONS ; HYBRIDIZATION ; OLIGONUCLEOTIDE ; AMPLIFICATION ; MICROCHIPS ; SYSTEMS ; FLOW
英文摘要Rapid screening of phenylketonuria using a CD microfluidic device; We herein present a compact disc (CD) microfluidic chip based hybridization assay for phenylketonuria (PKU) screening. This CD chip is composed of a polydimethylsiloxane (PDMS) top layer containing 12 DNA hybridization microchannels, and a glass bottom layer with hydrogel pad conjugated DNA oligonucleotides. Reciprocating flow was generated on the CD chip through a simple rotation-pause operation to facilitate rapid DNA hybridization. When rotated the CD chip, the sample solution was driven into the hybridization channel by centrifugal force. When stopped the CD chip, the sample plug was pulled backward through the channel by capillary force. The hybridization assay was firstly validated with control samples and was then used to analyze 30 clinical samples from pregnant women with suspected PKU fetus. The on-chip DNA hybridization was completed in 15 min with a sample consumption as low as 1.5 mu L, and the limit-of-detection (LOD) of DNA template was 0.7 ng/mu L Among the 30 samples tested, V245V mutation was identified in 4 cases while R243Q mutation was detected in one case. Results of the hybridization assay were confirmed by DNA sequencing. This CD-chip based hybridization assay features short analysis time, simple operation and low cost, thus has the potential to serve as the tool for PKU screening. (C) 2011 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000289133800014
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/115293
专题中国科学院大连化学物理研究所
通讯作者XiaomianZhou; DayuLiu
作者单位1.Guangzhou Med Coll, Guangzhou Municipal Peoples Hosp 1, Dept Lab Med, Guangzhou 510180, Guangdong, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Liaoning, Peoples R China
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GB/T 7714
Chen, Bin,Zhou, Xiaomian,Li, Chunyu,et al. Rapid screening of phenylketonuria using a CD microfluidic device[J]. JOURNAL OF CHROMATOGRAPHY A,2011,1218(14):1907-1912.
APA Chen, Bin.,Zhou, Xiaomian.,Li, Chunyu.,Wang, Qiuping.,Liu, Dayu.,...&DayuLiu.(2011).Rapid screening of phenylketonuria using a CD microfluidic device.JOURNAL OF CHROMATOGRAPHY A,1218(14),1907-1912.
MLA Chen, Bin,et al."Rapid screening of phenylketonuria using a CD microfluidic device".JOURNAL OF CHROMATOGRAPHY A 1218.14(2011):1907-1912.
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