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学科主题: 物理化学
题名: Rapid screening of phenylketonuria using a CD microfluidic device
作者: Chen, Bin1;  Zhou, Xiaomian1;  Li, Chunyu2;  Wang, Qiuping1;  Liu, Dayu1;  Lin, Bingcheng2
通讯作者: XiaomianZhou ;  DayuLiu
关键词: Phenylketonuria ;  DNA hybridization ;  CD microfluidic chip ;  Reciprocating flow
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2011-04-08
DOI: 10.1016/j.chroma.2011.02.001
卷: 1218, 期:14, 页:1907-1912
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 2,3
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
摘要: Rapid screening of phenylketonuria using a CD microfluidic device
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: We herein present a compact disc (CD) microfluidic chip based hybridization assay for phenylketonuria (PKU) screening. This CD chip is composed of a polydimethylsiloxane (PDMS) top layer containing 12 DNA hybridization microchannels, and a glass bottom layer with hydrogel pad conjugated DNA oligonucleotides. Reciprocating flow was generated on the CD chip through a simple rotation-pause operation to facilitate rapid DNA hybridization. When rotated the CD chip, the sample solution was driven into the hybridization channel by centrifugal force. When stopped the CD chip, the sample plug was pulled backward through the channel by capillary force. The hybridization assay was firstly validated with control samples and was then used to analyze 30 clinical samples from pregnant women with suspected PKU fetus. The on-chip DNA hybridization was completed in 15 min with a sample consumption as low as 1.5 mu L, and the limit-of-detection (LOD) of DNA template was 0.7 ng/mu L Among the 30 samples tested, V245V mutation was identified in 4 cases while R243Q mutation was detected in one case. Results of the hybridization assay were confirmed by DNA sequencing. This CD-chip based hybridization assay features short analysis time, simple operation and low cost, thus has the potential to serve as the tool for PKU screening. (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: PHENYLALANINE-HYDROXYLASE GENE ;  POLYMERASE-CHAIN-REACTION ;  DNA ;  MUTATIONS ;  HYBRIDIZATION ;  OLIGONUCLEOTIDE ;  AMPLIFICATION ;  MICROCHIPS ;  SYSTEMS ;  FLOW
语种: 英语
WOS记录号: WOS:000289133800014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115293
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Guangzhou Med Coll, Guangzhou Municipal Peoples Hosp 1, Dept Lab Med, Guangzhou 510180, Guangdong, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Liaoning, Peoples R China

Recommended Citation:
Chen, Bin,Zhou, Xiaomian,Li, Chunyu,et al. Rapid screening of phenylketonuria using a CD microfluidic device[J]. JOURNAL OF CHROMATOGRAPHY A,2011,1218(14):1907-1912.
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