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学科主题: 物理化学
题名: Perspectives of Comprehensive Phosphoproteome Analysis Using Shotgun Strategy
作者: Wang, Fangjun1;  Song, Chunxia1;  Cheng, Kai1;  Jiang, Xinning1;  Ye, Mingliang1;  Zou, Hanfa1
通讯作者: 邹汉法
刊名: ANALYTICAL CHEMISTRY
发表日期: 2011-11-01
DOI: 10.1021/ac201833j
卷: 83, 期:21, 页:8078-8085
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1,1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
摘要: Perspectives of Comprehensive Phosphoproteome Analysis Using Shotgun Strategy
研究领域[WOS]: Chemistry
英文摘要: Protein phosphorylation is a ubiquitous post-translational modification that regulates almost all cellular processes. The analysis, of protein phosphorylation is challenging due to the high dynamic range and low abundance natures of the analyte. Shotgun based proteomics has emerged as a very useful platform to achieve a comprehensive phosphoproteome analysis in considerable depth. In the past few years, significant breakthroughs on the large scale phosphorylation analysis have been witnessed along with the great development of related technologies. The combination of effective enrichment materials, refined analysis workflows, new type of powerful mass spectrometers, and sophisticated bioinformatic tools greatly boost the performance of. comprehensive phosphoproteome analysis. In this Perspective, we briefly reviewed recent technological developments on the enrichment materials, prefractionation workflows, and different Mass spectrometry fragmentation modes as well as software tools for phosphoproteome identification and quantification. Then, we described the current challenges and potential directions for the future of comprehensive phosphoproteome analysis. We also provide perspectives on how to further improve the performance of related analysis methods and technologies.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ;  MALDI-TOF MS ;  PROTEIN IDENTIFICATION TECHNOLOGY ;  ION AFFINITY-CHROMATOGRAPHY ;  HIGHLY SELECTIVE ENRICHMENT ;  QUANTITATIVE PHOSPHOPROTEOMICS ;  PHOSPHORYLATED PEPTIDES ;  SITE LOCALIZATION ;  TITANIUM-DIOXIDE ;  PHOSPHOPEPTIDE IDENTIFICATIONS
语种: 英语
WOS记录号: WOS:000296225300008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115300
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Wang, Fangjun,Song, Chunxia,Cheng, Kai,et al. Perspectives of Comprehensive Phosphoproteome Analysis Using Shotgun Strategy[J]. ANALYTICAL CHEMISTRY,2011,83(21):8078-8085.
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