DICP OpenIR
Subject Area物理化学
Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides
Wang, Hui1,2; Duan, Jicheng1,2; Xu, Hongjiu3; Zhao, Liang1,2; Liang, Yu1,2; Shan, Yichu1; Zhang, Lihua1; Liang, Zhen1; Zhang, Yukui1; Zhang LH(张丽华)
KeywordImmobilized Zirconium Ion Affinity Chromatography Monolith Phosphopeptide Photografting Photopolymerization Selective Enrichment
Source PublicationJOURNAL OF SEPARATION SCIENCE
2011-09-01
ISSN待补充
DOI10.1002/jssc.201100168
Volume34Issue:16-17Pages:2113-2121
Indexed BySCI
SubtypeArticle
Department18
Funding Project1810
Contribution Rank1,1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordMETAL AFFINITY-CHROMATOGRAPHY ; CAPILLARY LIQUID-CHROMATOGRAPHY ; POROUS POLYMER MONOLITHS ; PHOSPHOPROTEOME ANALYSIS ; PHOSPHORYLATED PEPTIDES ; MASS-SPECTROMETRY ; MICROFLUIDIC CHIPS ; SURFACE-CHEMISTRY ; COLUMNS ; ELECTROCHROMATOGRAPHY
AbstractMonoliths with immobilized zirconium ions for selective enrichment of phosphopeptides; To meet the demands of protein phosphorylation study, immobilized zirconium ion affinity chromatography (Zr4+-IMAC) monolith was prepared by combining UV-initiated polymerization of monolithic support and subsequent photografting in both capillary columns and microchannels. Hydrophilic poly(2-hydroxyethyl methacrylate (HEMA)-co-ethylene dimethacrylate (EDMA)) monolithic support was prepared under UV irradiation at the wavelength of 365 nm with monomer HEMA, crosslinker EDMA and 2,2-dimethoxy-2-phenylacetophenone as photoinitiator in 1-decanol solution, which provides good biocompatibility and permeability for biomolecule analysis. To introduce chelating ligands, such as phosphate groups, on the pore surface of monolith for metal ion immobilization, photografting of ethylene glycol methacrylate phosphate with benzophenone as the photoinitiator was performed at 254 nm for 300 s. The grafting process and metal ion immobilization can be monitored by measuring the electroosmotic flow produced by the modified monolith, providing a quantitative evaluation of post-modification. This new method for the preparation of Zr4+-IMAC monolith simplifies the optimization of monolith preparation and avoids the time-consuming chemical modification process. Additionally, advantages include facile preparation in microdevices, easy regenerability and good reproducibility. After optimization, the microchip-based Zr4+-IMAC monolith was used for phosphopeptide analysis and showed good selectivity in phosphopeptide enrichment with matrix-assisted laser desorption ionization mass spectrometry detection.
Language英语
WOS IDWOS:000295189400019
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/115303
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang LH(张丽华)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 1, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Wang, Hui,Duan, Jicheng,Xu, Hongjiu,et al. Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(16-17):2113-2121.
APA Wang, Hui.,Duan, Jicheng.,Xu, Hongjiu.,Zhao, Liang.,Liang, Yu.,...&张丽华.(2011).Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides.JOURNAL OF SEPARATION SCIENCE,34(16-17),2113-2121.
MLA Wang, Hui,et al."Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides".JOURNAL OF SEPARATION SCIENCE 34.16-17(2011):2113-2121.
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