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学科主题: 物理化学
题名: Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides
作者: Wang, Hui1, 2;  Duan, Jicheng1, 2;  Xu, Hongjiu3;  Zhao, Liang1, 2;  Liang, Yu1, 2;  Shan, Yichu1;  Zhang, Lihua1;  Liang, Zhen1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Immobilized zirconium ion affinity chromatography ;  Monolith ;  Phosphopeptide ;  Photografting ;  Photopolymerization ;  Selective enrichment
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2011-09-01
DOI: 10.1002/jssc.201100168
卷: 34, 期:16-17, 页:2113-2121
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1,1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
摘要: Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides
研究领域[WOS]: Chemistry
英文摘要: To meet the demands of protein phosphorylation study, immobilized zirconium ion affinity chromatography (Zr4+-IMAC) monolith was prepared by combining UV-initiated polymerization of monolithic support and subsequent photografting in both capillary columns and microchannels. Hydrophilic poly(2-hydroxyethyl methacrylate (HEMA)-co-ethylene dimethacrylate (EDMA)) monolithic support was prepared under UV irradiation at the wavelength of 365 nm with monomer HEMA, crosslinker EDMA and 2,2-dimethoxy-2-phenylacetophenone as photoinitiator in 1-decanol solution, which provides good biocompatibility and permeability for biomolecule analysis. To introduce chelating ligands, such as phosphate groups, on the pore surface of monolith for metal ion immobilization, photografting of ethylene glycol methacrylate phosphate with benzophenone as the photoinitiator was performed at 254 nm for 300 s. The grafting process and metal ion immobilization can be monitored by measuring the electroosmotic flow produced by the modified monolith, providing a quantitative evaluation of post-modification. This new method for the preparation of Zr4+-IMAC monolith simplifies the optimization of monolith preparation and avoids the time-consuming chemical modification process. Additionally, advantages include facile preparation in microdevices, easy regenerability and good reproducibility. After optimization, the microchip-based Zr4+-IMAC monolith was used for phosphopeptide analysis and showed good selectivity in phosphopeptide enrichment with matrix-assisted laser desorption ionization mass spectrometry detection.
关键词[WOS]: METAL AFFINITY-CHROMATOGRAPHY ;  CAPILLARY LIQUID-CHROMATOGRAPHY ;  POROUS POLYMER MONOLITHS ;  PHOSPHOPROTEOME ANALYSIS ;  PHOSPHORYLATED PEPTIDES ;  MASS-SPECTROMETRY ;  MICROFLUIDIC CHIPS ;  SURFACE-CHEMISTRY ;  COLUMNS ;  ELECTROCHROMATOGRAPHY
语种: 英语
WOS记录号: WOS:000295189400019
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115303
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 1, Dalian, Peoples R China

Recommended Citation:
Wang, Hui,Duan, Jicheng,Xu, Hongjiu,et al. Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(16-17):2113-2121.
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