DICP OpenIR
学科主题物理化学
Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides
Wang, Hui1,2; Duan, Jicheng1,2; Xu, Hongjiu3; Zhao, Liang1,2; Liang, Yu1,2; Shan, Yichu1; Zhang, Lihua1; Liang, Zhen1; Zhang, Yukui1; Zhang LH(张丽华)
关键词Immobilized Zirconium Ion Affinity Chromatography Monolith Phosphopeptide Photografting Photopolymerization Selective Enrichment
刊名JOURNAL OF SEPARATION SCIENCE
2011-09-01
ISSN待补充
DOI10.1002/jssc.201100168
34期:16-17页:2113-2121
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名1,1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]METAL AFFINITY-CHROMATOGRAPHY ; CAPILLARY LIQUID-CHROMATOGRAPHY ; POROUS POLYMER MONOLITHS ; PHOSPHOPROTEOME ANALYSIS ; PHOSPHORYLATED PEPTIDES ; MASS-SPECTROMETRY ; MICROFLUIDIC CHIPS ; SURFACE-CHEMISTRY ; COLUMNS ; ELECTROCHROMATOGRAPHY
英文摘要Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides; To meet the demands of protein phosphorylation study, immobilized zirconium ion affinity chromatography (Zr4+-IMAC) monolith was prepared by combining UV-initiated polymerization of monolithic support and subsequent photografting in both capillary columns and microchannels. Hydrophilic poly(2-hydroxyethyl methacrylate (HEMA)-co-ethylene dimethacrylate (EDMA)) monolithic support was prepared under UV irradiation at the wavelength of 365 nm with monomer HEMA, crosslinker EDMA and 2,2-dimethoxy-2-phenylacetophenone as photoinitiator in 1-decanol solution, which provides good biocompatibility and permeability for biomolecule analysis. To introduce chelating ligands, such as phosphate groups, on the pore surface of monolith for metal ion immobilization, photografting of ethylene glycol methacrylate phosphate with benzophenone as the photoinitiator was performed at 254 nm for 300 s. The grafting process and metal ion immobilization can be monitored by measuring the electroosmotic flow produced by the modified monolith, providing a quantitative evaluation of post-modification. This new method for the preparation of Zr4+-IMAC monolith simplifies the optimization of monolith preparation and avoids the time-consuming chemical modification process. Additionally, advantages include facile preparation in microdevices, easy regenerability and good reproducibility. After optimization, the microchip-based Zr4+-IMAC monolith was used for phosphopeptide analysis and showed good selectivity in phosphopeptide enrichment with matrix-assisted laser desorption ionization mass spectrometry detection.
语种英语
WOS记录号WOS:000295189400019
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/115303
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 1, Dalian, Peoples R China
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Wang, Hui,Duan, Jicheng,Xu, Hongjiu,et al. Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(16-17):2113-2121.
APA Wang, Hui.,Duan, Jicheng.,Xu, Hongjiu.,Zhao, Liang.,Liang, Yu.,...&张丽华.(2011).Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides.JOURNAL OF SEPARATION SCIENCE,34(16-17),2113-2121.
MLA Wang, Hui,et al."Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides".JOURNAL OF SEPARATION SCIENCE 34.16-17(2011):2113-2121.
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