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学科主题: 物理化学
题名: On-line combination of monolithic immobilized pH gradient-based capillary isoelectric focusing and capillary zone electrophoresis via a partially etched porous interface for protein analysis
作者: Wang, Tingting1, 2;  Ma, Junfeng1;  Wu, Shuaibin1, 2;  Sun, Liangliang1, 2;  Yuan, Huiming1;  Zhang, Lihua1;  Liang, Zhen1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Two-dimensional capillary electrophoresis ;  Monolithic immobilized pH gradient-based capillary isoelectric focusing ;  Capillary zone electrophoresis ;  Partially etched porous interface ;  Protein
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2011-04-01
DOI: 10.1016/j.jchromb.2011.02.020
卷: 879, 期:11-12, 页:804-810
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
摘要: On-line combination of monolithic immobilized pH gradient-based capillary isoelectric focusing and capillary zone electrophoresis via a partially etched porous interface for protein analysis
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: An integrated platform consisting of monolithic immobilized pH gradient-based capillary isoelectric focusing (M-IPG CIEF) and capillary zone electrophoresis (CZE) coupled by a partially etched porous interface was established. Since carrier ampholytes (CAs) were immobilized on monolith in M-IPG CIEF to form a stable pH gradient, subsequent depletion of CAs at the interface to prevent the interference on CZE separation and detection were avoided. Moreover, a partially etched porous capillary column, which was facile for fabrication and durable for operation, was exploited as the interface to combine M-IPG CIEF and CZE. The RSD values in terms of the migration time for M-IPG CIEF separation, transfer protein from the first dimension to the second dimension, and CZE separation, were 2.4%, 3.9% and 2.3%, respectively. With a 6-protein mixture as the sample, two-dimensional capillary electrophoresis (2D-CE) separation was successfully completed within 116 min, yielding a peak capacity of similar to 200 even with minute sample amount down to 5.0 mu g/mL. The limit of detection was 0.2 mu g/mL. In addition, proteins extracted from milk were used to test the performance of such a 2D-CE separation platform. We expect that such a novel 20-CE system would provide a promising tool for protein separation with high throughput and high peak capacity. (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: 2-DIMENSIONAL SEPARATION SYSTEM ;  MASS-SPECTROMETRY ;  GEL-ELECTROPHORESIS ;  PROTEOMICS ;  COLUMN ;  JOINT ;  IDENTIFICATION ;  CHROMATOGRAPHY ;  INTEGRATION ;  PEPTIDES
语种: 英语
WOS记录号: WOS:000289382700018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115309
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Wang, Tingting,Ma, Junfeng,Wu, Shuaibin,et al. On-line combination of monolithic immobilized pH gradient-based capillary isoelectric focusing and capillary zone electrophoresis via a partially etched porous interface for protein analysis[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(11-12):804-810.
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