中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: Ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics
作者: Sun, Liangliang1, 2;  Tao, Dingyin1, 2;  Han, Bin1;  Ma, Junfeng1;  Zhu, Guijie1;  Liang, Zhen1;  Shan, Yichu1;  Zhang, Lihua1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Ionic liquid ;  1-Butyl-3-methyl imidazolium tetrafluoroborate ;  Integral membrane proteins ;  Shotgun
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2011-04-01
DOI: 10.1007/s00216-010-4381-5
卷: 399, 期:10, 页:3387-3397
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
摘要: Ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The solubility and digestion efficiency are two crucial factors that might affect the identification of integral membrane proteins (IMPs). In this work, 1% (v/v) ionic liquid (IL), 1-butyl-3-methyl imidazolium tetrafluoroborate (BMIM BF(4)), added in NH(4)HCO(3) buffer (pH 8.3), was applied as a sample preparation buffer for IMPs analysis. Compared to the commonly used sodium dodecyl sulfate and methanol methods, the number of identified IMPs from rat brain by microcolumn reversed phase liquid chromatography (mu RPLC)-electrospray ionization tandem mass spectrometry (ESI-MS/MS) was improved by over three times, which might be due to the fact that BMIM BF(4) offered high solubilizing ability for IMPs and good compatibility for tryptic digestion. Furthermore, compared to Rapigest and urea methods, with BMIM BF(4) method, the number of identified IMPs from rat brain could be improved 25% and 80%, respectively, which might be contributed to the good solubilizing ability and high thermal stability of such IL. With the sample treated by BMIM BF(4) method, by 2D-nanoSCX-RPLC-ESI-MS/MS, 1,450 non-redundant proteins and 7,978 unique peptides were identified from rat brain, and 418 proteins contained at least one predicted transmembrane domain, with false discovery rates of less than 1% for peptide identification, and at least two identified unique peptides per protein. All these results demonstrate that the BMIM BF(4) method is of high potential for the large-scale identification of IMPs.
关键词[WOS]: DESORPTION/IONIZATION MASS-SPECTROMETRY ;  LC-MS/MS ;  TRYPSIN DIGESTION ;  PLASMA-MEMBRANE ;  LARGE-SCALE ;  PROTEINS ;  EXTRACTION ;  AMINO ;  PH ;  IDENTIFICATION
语种: 英语
WOS记录号: WOS:000288398700008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115311
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2011Xs82HtDqlw.pdf(386KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Sun, Liangliang,Tao, Dingyin,Han, Bin,et al. Ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,399(10):3387-3397.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Sun, Liangliang]'s Articles
 [Tao, Dingyin]'s Articles
 [Han, Bin]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Sun, Liangliang]‘s Articles
 [Tao, Dingyin]‘s Articles
 [Han, Bin]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2011Xs82HtDqlw.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace