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学科主题: 物理化学
题名: Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity
作者: Zhang, Liyuan1, 2;  Xu, Jin3;  Sun, Liangliang1, 2;  Ma, Junfeng1, 2;  Yang, Kaiguang1;  Liang, Zhen1;  Zhang, Lihua1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: ZrO(2) aerogel ;  Phosphopeptide ;  Enrichment ;  Mass spectrometry
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2011-04-01
DOI: 10.1007/s00216-011-4657-4
卷: 399, 期:10, 页:3399-3405
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
摘要: Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: In this study, zirconium oxide (ZrO(2)) aerogel was synthesized via a green sol-gel approach, with zirconium oxychloride, instead of the commonly used alkoxide with high toxicity, as the precursor. With such material, phosphopeptides from the digests of 4 pmol of beta-casein with the coexistence of 100 times (mol ratio) BSA could be selectively captured, and identified by MALDI-TOF MS. Due to the large surface area (416.0 m(2) g(-1)) and the mesoporous structure (the average pore size of 10.2 nm) of ZrO(2) aerogel, a 20-fold higher loading capacity for phosphopeptide, YKVPQLEIVPN[pS]AEER (MW 1952.12), was obtained compared to that of commercial ZrO(2) microspheres (341.5 vs. 17.87 mg g(-1)). The metal oxide aerogel was further applied in the enrichment of phosphopeptides from 100 ng nonfat milk, and 17 phosphopeptides were positively identified, with a 1.5-fold improvement in phosphopeptide detection compared with previously reported results. These results demonstrate that ZrO(2) aerogel can be a powerful enrichment material for phosphoproteome study.
关键词[WOS]: ION AFFINITY-CHROMATOGRAPHY ;  TIN DIOXIDE MICROSPHERES ;  PHOSPHORYLATED PEPTIDES ;  MASS-SPECTROMETRY ;  PHOSPHOPROTEOME ANALYSIS ;  NANOPARTICLES ;  PERFORMANCE ;  ADSORBENT ;  GROWTH ;  CARBON
语种: 英语
WOS记录号: WOS:000288398700009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115312
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.China Med Univ, Shengjing Hosp, Dept Hepat Biliary Pancreas & Transplantat Surg, Shenyang 110004, Peoples R China

Recommended Citation:
Zhang, Liyuan,Xu, Jin,Sun, Liangliang,et al. Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,399(10):3399-3405.
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