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学科主题物理化学
Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity
Zhang, Liyuan1,2; Xu, Jin3; Sun, Liangliang1,2; Ma, Junfeng1,2; Yang, Kaiguang1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1; Zhang LH(张丽华)
关键词Zro(2) Aerogel Phosphopeptide Enrichment Mass Spectrometry
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
2011-04-01
ISSN待补充
DOI10.1007/s00216-011-4657-4
399期:10页:3399-3405
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名1,1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]ION AFFINITY-CHROMATOGRAPHY ; TIN DIOXIDE MICROSPHERES ; PHOSPHORYLATED PEPTIDES ; MASS-SPECTROMETRY ; PHOSPHOPROTEOME ANALYSIS ; NANOPARTICLES ; PERFORMANCE ; ADSORBENT ; GROWTH ; CARBON
英文摘要Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity; In this study, zirconium oxide (ZrO(2)) aerogel was synthesized via a green sol-gel approach, with zirconium oxychloride, instead of the commonly used alkoxide with high toxicity, as the precursor. With such material, phosphopeptides from the digests of 4 pmol of beta-casein with the coexistence of 100 times (mol ratio) BSA could be selectively captured, and identified by MALDI-TOF MS. Due to the large surface area (416.0 m(2) g(-1)) and the mesoporous structure (the average pore size of 10.2 nm) of ZrO(2) aerogel, a 20-fold higher loading capacity for phosphopeptide, YKVPQLEIVPN[pS]AEER (MW 1952.12), was obtained compared to that of commercial ZrO(2) microspheres (341.5 vs. 17.87 mg g(-1)). The metal oxide aerogel was further applied in the enrichment of phosphopeptides from 100 ng nonfat milk, and 17 phosphopeptides were positively identified, with a 1.5-fold improvement in phosphopeptide detection compared with previously reported results. These results demonstrate that ZrO(2) aerogel can be a powerful enrichment material for phosphoproteome study.
语种英语
WOS记录号WOS:000288398700009
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/115312
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
作者单位1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.China Med Univ, Shengjing Hosp, Dept Hepat Biliary Pancreas & Transplantat Surg, Shenyang 110004, Peoples R China
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GB/T 7714
Zhang, Liyuan,Xu, Jin,Sun, Liangliang,et al. Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,399(10):3399-3405.
APA Zhang, Liyuan.,Xu, Jin.,Sun, Liangliang.,Ma, Junfeng.,Yang, Kaiguang.,...&张丽华.(2011).Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,399(10),3399-3405.
MLA Zhang, Liyuan,et al."Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 399.10(2011):3399-3405.
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